The Histoplasma capsulatum cdc2 gene is transcriptionally regulated during the morphologic transition.
The Histoplasma capsulatum cdc2 gene is transcriptionally regulated during the morphologic transition.
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荚膜组织胞浆菌 cdc2 基因在形态转变过程中受到转录调控。
DOI：
10.1016/0378-1119(94)90729-3
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Maresca,B
中科院分区：
文献类型：
--
作者：
DiLallo,G;Gargano,S;Maresca,B
为了了解控制二相性真菌从菌丝到酵母的可逆形态转变的分子机制，我们从被膜组织胞浆菌中分离并鉴定了acdc2基因。这种微生物是一种二相性致病真菌，在土壤中以丝状腐生霉菌的形式生长，在人体组织中以单细胞致病酵母的形式生长。克隆的基因，其蛋白产物与dc2家族的其他成员高度同源，被分成四个外显子和三个内含子，分别为95、52和85个核苷酸。对克隆的分析证实了真核剪接供体(GT)和受体(AG)位点的存在。该拼接基因编码324个氨基酸(Aa)的蛋白质，预测分子质量为36.9 kDa。他们。胶囊cdc2产物与酿酒酵母的氨基酸同源性为71%，与裂殖酵母的同源性为70%。推导出的蛋白含有通常在大多数p34cdc2蛋白中发现的序列PSTAIRE。胶囊H.encatumcdc2在菌丝体酵母形态转变过程中受到转录调控，并且在酵母中的转录比在菌丝期更活跃。
To understand the molecular mechanisms that control the reversible morphologic transition from mycelia to yeast in dimorphic fungi, we have isolated and characterized acdc2gene fromHistoplasma capsulatum. This organism is a dimorphic pathogenic fungus that grows as a filamentous saprobic mold in soil and as a unicellular pathogenic yeast in human tissue. The cloned gene, whose protein product has a high degree of homology with other members of thecdc2family, is split into four exons and three introns of 95, 52 and 85 nucleotides. Analyses of cDNA clones confirm the presence of the eukaryotic splice donor (GT) and acceptor (AG) sites. The spliced gene codes for a protein of 324 amino acids (aa) with a predicted molecular mass of 36.9 kDa. TheH. capsulatum cdc2product has 71% aa identity withSaccharomyces cerevisiaeand 70% withSchizosaccharomyces pombe. The deduced protein contains the sequence, PSTAIRE, that is normally found in most p34cdc2proteins.H. capsulatum cdc2is transcriptionally regulated during the morphologic mycelium yeast transitions and is more actively transcribed in the yeast than in the mycelial phase.