Resampling and deconvolution of linear time-of-flight records for enhanced protein profiling.
Resampling and deconvolution of linear time-of-flight records for enhanced protein profiling.
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对线性飞行时间记录进行重采样和反卷积以增强蛋白质分析。
DOI：
10.1002/rcm.2496
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Manos,DennisM
中科院分区：
文献类型：
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作者：
Malyarenko,DariyaI;Cooke,WilliamE;Tracy,EugeneR;Drake,RichardR;Shin,Susanna;Semmes,OJohn;Sasinowski,Maciek;Manos,DennisM
我们开发了一种适用于飞行时间（TOF）光谱的全质量范围的峰值去卷积策略。该策略涉及重新扫描光谱以创建在整个光谱上具有相等峰宽（在时间上）的时间序列，然后使用我们先前描述的去卷积滤波器。我们使用这种技术去卷积的蛋白质质谱血清和细胞裂解物上获得三个独立的TOF仪器。在去卷积之后，我们解析出与预期事件一致的光谱结构，例如多电荷离子、基质加合物和翻译后蛋白质修饰。反褶积过程在线性TOF记录的整个长度上产生40%的分辨率提高和增强的实验灵敏度，高达tom/z 150 000。该方法特别适用于密集TOF谱中的自动数据分析和峰检测。版权所有© 2006约翰威利父子有限公司。
We have developed a peak deconvolution strategy that is applicable to the full mass range of a time‐of‐flight (TOF) spectrum. This strategy involves resampling a spectrum to create a time series that has equal peak widths (in time) across the entire spectrum, and then using the deconvolution filters we have previously described. We use this technique to deconvolve the protein mass spectra for blood serum and cell lysates acquired on three separate TOF instruments. Following deconvolution, we resolve spectral structures consistent with expected events such as multiply charged ions, matrix adducts and post‐translational protein modifications. The deconvolution procedure produces a 40% improvement in the resolution and enhanced experimental sensitivity over the full length of the linear TOF record, up tom/z150 000. This approach is particularly appropriate for automated data analysis and peak detection in dense TOF spectra. Copyright © 2006 John Wiley & Sons, Ltd.