Meniscal allograft transplantation in the sheep knee - Evaluation of chondroprotective effects
Meniscal allograft transplantation in the sheep knee - Evaluation of chondroprotective effects
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DOI：
10.1177/0363546506287365
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发表时间：
2006-09-01
影响因子：
4.8
通讯作者：
Rodeo, Scott A.
中科院分区：
文献类型：
--
作者：
Kelly, Bryan T.;Potter, Hollis G.;Rodeo, Scott A.
背景资料：在X线片上出现退行性变化之前对关节软骨进行早期保护，应该会产生更好的长期效果，但缺乏关于关节移植有效性的科学证据。目的：评估一种新的同种异体骨移植动物模型的软骨保护作用，并评估磁共振成像参数T2标测，研究设计：对照实验室研究方法：将45只睾丸成熟的绵羊分为3个手术组：侧髂动脉切除术（n = 24），髂动脉同种异体移植（n = 17）和假手术（n = 4）。在2、4或12个月时处死动物。通过大体检查、磁共振成像、T2标测、生物力学测试和半定量组织学分析评估腕关节。与对照肢体相比，所有客观标准显示，椎间盘切除术导致软骨退变显著增加（P <0.01）。同种异体髂骨移植术后软骨退变明显减轻（P <0.02）。T2标测数据与所有其他传统结局指标之间存在显著相关性（P <0.05，r（2）= 0.37-0.67）。与未手术的对照肢体相比，同种异体移植物在2个月时除磁共振成像数据外，其他任何类别均无显著差异。到4个月，nonoperated控制肢体表现出显着减少磨损相比，同种异体移植肢体在所有类别中，除了修改Mankin scores.Conclusion：该模型表现出显着的软骨保护相比，anastrocectomy，但表现出更多的软骨磨损在4个月相比，控制肢体。在同种异体移植物中观察到高度的同种异体移植物细胞活力和血管长入。T2映射可以提供一个准确的非侵入性措施，早期软骨退变后，tumercectomy，以及软骨保护后tumercal allograft transplantation.Clinical Relevance：这项研究建立了一个可靠的动物模型tumercal allograft transplantation和提供证据的效用T2映射在临床相关的磁共振成像场强评估早期软骨退变。
Background: Early protection of articular cartilage, before degenerative changes appear on radiographs, should result in better long-term results, but scientific evidence regarding the effectiveness of meniscal transplantation is lacking.Purpose: To assess the chondroprotective effects of a new meniscal allograft transplantation animal model and evaluate a magnetic resonance imaging parameter, T2 mapping, in articular cartilage after meniscectomy and meniscal transplantation.Study Design: Controlled laboratory study.Methods: Forty-five skeletally mature sheep were placed into 3 surgical groups: lateral meniscectomy (n = 24), meniscal allograft transplant (n = 17), and sham (n = 4). Animals were sacrificed at 2, 4, or 12 months. Cartilage was assessed by gross inspection, magnetic resonance imaging, T2 mapping, biornechanical testing, and semiquantitative histologic analysis.Results: There were no differences between the sham operation and nonoperated control limbs. Compared with control limbs, meniscectomy resulted in significant increases in cartilage degeneration by all objective criteria (P < .01). Compared with meniscectomy, meniscal allograft transplantation resulted in significant decreases in cartilage degeneration (P < .02). There were significant correlations between T2-mapping data and all other traditional outcomes measures (P < .05, r(2) = 0.37-0.67). Compared with the nonoperated control limbs, allograft transplants demonstrated no significant differences at 2 months in any category, except magnetic resonance imaging data. By 4 months, nonoperated control limbs demonstrated significantly less wear compared to allograft limbs in all categories except modified Mankin scores.Conclusion: This model demonstrated significant chondroprotection compared with meniscectomy but demonstrated more cartilage wear at 4 months compared to control limbs. A high degree of allograft cell viability and vascular ingrowth was seen in allograft explants. T2 mapping may provide an accurate noninvasive measure of early cartilage degeneration after meniscectomy, as well as cartilage protection after meniscal allograft transplantation.Clinical Relevance: This study establishes a reliable animal model for meniscal allograft transplantation and provides evidence for the utility of T2 mapping at clinically relevant magnetic resonance imaging field strengths for evaluation of early cartilage degeneration.