Crosstalk between histological alterations, oxidative stress and immune aberrations of the emerging PFOS alternative OBS in developing zebrafish
Crosstalk between histological alterations, oxidative stress and immune aberrations of the emerging PFOS alternative OBS in developing zebrafish
复制标题
新兴 PFOS 替代 OBS 在斑马鱼发育中的组织学改变、氧化应激和免疫畸变之间的串扰
DOI：
10.1016/j.scitotenv.2021.145443
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发表时间：
2021-02-19
影响因子：
9.8
通讯作者：
Tu, Wenqing
中科院分区：
文献类型：
--
作者：
Huang, Jing;Wang, Qiyu;Tu, Wenqing
对全氟壬烯氧基苯磺酸钠（OBS）作为一种新型的全氟辛烷磺酸（PFOS）替代品在许多工业领域得到了广泛的应用。然而，我们对其毒性作用的了解是有限的。在这项研究中，通过检查PFOS和OBS对组织学、氧化应激和免疫反应的影响，比较了它们对斑马鱼幼鱼的毒性。在相同的暴露浓度下，OBS引起的死亡率和畸形率低于PFOS。然而，全氟辛烷磺酸和OBS均引起组织学改变，分别表现为体横纹肌细胞坏死和肠褶高度降低。在PFOS和OBS处理组中分别观察到MDA和ROS含量的显著增加，表明斑马鱼幼体中发生了氧化应激。转录组学和qRT-PCR结果显示，PFOS和OBS处理组的大多数免疫相关基因表达均上调，但后者上调幅度较小。与它们的mRNA表达一致，IL- 1 β、CXCL 8、MMP 9和Casp 8蛋白的表达在OBS暴露后显著升高。然而，这些蛋白质的表达没有受到全氟辛烷磺酸的影响，可能是因为翻译过程受到严重干扰。总体而言，OBS和PFOS均可诱导斑马鱼幼鱼产生氧化应激和炎症反应。我们的研究结果强调，不应忽视OBS作为全氟辛烷磺酸替代品的健康风险。(c)2021爱思唯尔有限公司版权所有。
Sodium p-perfluorous nonenoxybenzene sulfonate (OBS) has been widely applied as a novel PFOS alternative in many industrial fields. However, our knowledge about its toxic effects is limited. In this study, the toxicities of PFOS and OBS in zebrafish larvae were compared by examining their effects on histology, oxidative stress and immune responses. Mortality and malformations caused by OBS were lower than those caused by PFOS under the same exposure concentrations. However, both PFOS and OBS caused histological alterations, manifesting in somatic striated muscle cell necrosis and a reduction in intestinal fold height, respectively. Significant increases in MDA and ROS content were observed in the PFOS and OBS treatment groups, respectively, suggesting the occurrence of oxidative stress in the zebrafish larvae. Furthermore, the transcriptomic and qRT-PCR results showed that the expression ofmost immune-related genes was upregulated in both PFOS and OBS treatment groups, but the latterwas to a lesser extent. Consistent with their mRNA expression, the expression of the IL- 1 beta, CXCL8, MMP9 and Casp8 proteins was significantly elevated following OBS exposure. However, the expression of these proteins was not affected by PFOS, possibly because the translation process was severely disrupted. Collectively, both OBS and PFOS could induce oxidative stress and inflammatory responses in zebrafish larvae. Our findings highlight that the health risks of OBS as a substitute for PFOS should not be ignored. (c) 2021 Elsevier B.V. All rights reserved.