Inflammation and tissue damage in mouse lung by single and repeated dosing of urban air coarse and fine particles collected from six European cities
Inflammation and tissue damage in mouse lung by single and repeated dosing of urban air coarse and fine particles collected from six European cities
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DOI：
10.3109/08958370903527908
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发表时间：
2010-04-01
影响因子：
2.1
通讯作者：
Hirvonen, M. -R.
中科院分区：
文献类型：
--
作者：
Happo, M. S.;Salonen, R. O.;Hirvonen, M. -R.
作者先前已经证明了从六个欧洲城市收集的城市空气细颗粒物（PM2.5 - 0.2）和粗颗粒物（PM10 - 2.5）样本的炎症活动的异质性，这些城市的空气污染情况截然不同。在研究周的第1、3和6天，将相同的样品（10 mg/kg）一次或多次向健康C57 BL/6J小鼠进行气管内滴注。在单次给药后24小时或最后一次重复给药后灌洗肺。在这两个尺寸范围内，重复给药的颗粒增加了支气管肺泡灌洗液（BALF）中的总细胞数超过各自的单次给药，而细胞因子浓度在重复给药后较低。重复给药PM2.5 - 0.2样品后，乳酸脱氢酶（LDH）反应增加至2倍，重复给药PM10 - 2.5样品后增加至6倍。PM10 - 2.5样品在小鼠肺中诱发更广泛的间质炎症。对炎症反应有主要贡献的成分是PM2.5 - 0.2样品中的氧化有机化合物和过渡金属，PM10 - 2.5样品中的Cu和土壤矿物质，以及两种尺寸范围内的Zn。相比之下，差的生物质和煤炭燃烧与多环芳烃（PAHs）的水平升高，并对PM2.5 - 0.2样品的炎症活性具有一致的抑制作用。总之，与单次给药相比，细颗粒和粗颗粒样品的重复气管内滴注引起了增强的肺部炎症和细胞毒性。造成这些影响的城市空气颗粒物的来源和成分似乎与作者以前的单剂量研究中遇到的相似。
The authors have previously demonstrated heterogeneities in the inflammatory activities of urban air fine (PM2.5-0.2) and coarse (PM10-2.5) particulate samples collected from six European cities with contrasting air pollution situations. The same samples (10 mg/kg) were intratracheally instilled to healthy C57BL/6J mice either once or repeatedly on days 1, 3, and 6 of the study week. The lungs were lavaged 24 h after the single dose or after the last repeated dosing. In both size ranges, repeated dosing of particles increased the total cell number in bronchoalveolar lavage fluid (BALF) more than the respective single dose, whereas cytokine concentrations were lower after repeated dosing. The lactate dehydrogenase (LDH) responses increased up to 2-fold after repeated dosing of PM2.5-0.2 samples and up to 6-fold after repeated dosing of PM10-2.5 samples. PM10-2.5 samples evoked a more extensive interstitial inflammation in the mouse lungs. The constituents with major contributions to the inflammatory responses were oxidized organic compounds and transition metals in PM2.5-0.2 samples, Cu and soil minerals in PM10-2.5 samples, and Zn in both size ranges. In contrast, poor biomass and coal combustion were associated with elevated levels of polycyclic aromatic hydrocarbons (PAHs) and a consistent inhibitory effect on the inflammatory activity of PM2.5-0.2 samples. In conclusion, repeated intratracheal instillation of both fine and coarse particulate samples evoked enhanced pulmonary inflammation and cytotoxicity compared to single-dose administration. The sources and constituents of urban air particles responsible for these effects appear to be similar to those encountered in the authors' previous single-dose study.