Comparison of blood counts in venous, fingertip and arterial blood and their measurement variation
Comparison of blood counts in venous, fingertip and arterial blood and their measurement variation
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DOI：
10.1046/j.1365-2257.2001.00388.x
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发表时间：
2001-06-01
期刊：
影响因子：
--
通讯作者：
Tang, Z
中科院分区：
文献类型：
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作者：
Yang, ZW;Yang, SH;Tang, Z
我们比较了静脉血、指尖血和动脉血的血常规图像及其测量变化。同时采集24名志愿者的一对静脉和指尖血样，以及另外12名志愿者的一对静脉和动脉血样。这些志愿者都是健康的成年人，年龄在20-22岁之间，男女人数相等。每个人取三等分的血液，使用自动血液学分析仪进行三次血液计数测量，包括血红蛋白浓度和红细胞压积。与动脉血相比，静脉血中的红细胞计数（2.7%）和红细胞压积（3.1%）显着增加。指尖血白细胞总数和大白细胞计数明显高于静脉血（分别为9.2%和12.6%）。经反复取样，指尖血液中白细胞数量逐渐减少。指尖血或动脉血测量总白细胞或血红蛋白的测量间差异明显大于静脉血。静脉和动脉血中血红蛋白（1.3%）和小白细胞比率或血小板（约4.0%）的测量间变异系数分别最小和最大。微循环后动脉血浆体积减少2-3%。指尖血白细胞总数升高主要是由于粒细胞增多所致。静脉血比指尖血和动脉血测量精度高。
We compared routine blood pictures in venous, fingertip and arterial blood and their measurement variation. A pair of venous and fingertip blood samples were simultaneously collected from 24 volunteers, and another pair of venous and arterial samples from another 12 volunteers. The volunteers were healthy adults, aged 20-22 years, with males and females in equal numbers. Three aliquots of blood were taken from each person for three measurements of blood counts, including haemoglobin concentration and haemocrit, using an automatic haematology analyser, Compared to arterial blood, there was a significant increase of erythrocyte count (2.7%) and haematocrit (3.1%) in the venous blood. The total and large leucocyte counts were significantly higher (9.2% and 12.6%, respectively) in the fingertip blood than in the venous blood. There were a decreasing number of leucocytes in the fingertip blood with repeated sampling. The intermeasurement variation for total leucocyte or haemoglobin measurement was significantly larger as measured with the fingertip blood or arterial blood than with the venous blood. The intermeasurement coefficients of variation of haemoglobin (1.3%) and small leucocyte ratio or platelet (approximately 4.0%) measurements with venous and arterial blood were the smallest and largest, respectively. Volume of arterial plasma was diminished by 2-3% after microcirculation. Elevation of total leucocyte count in the fingertip blood was mainly due to the increase of granulocytes. Measurement with venous blood was of better precision than with fingertip or arterial blood.