trans-activation of viral enhancers including long terminal repeat of the human immunodeficiency virus by the hepatitis B virus X protein.
trans-activation of viral enhancers including long terminal repeat of the human immunodeficiency virus by the hepatitis B virus X protein.
复制标题
病毒增强子的反式激活，包括乙型肝炎病毒 X 蛋白对人类免疫缺陷病毒的长末端重复。
DOI：
10.1016/0042-6822(89)90177-3
复制
发表时间：
1989
期刊：
影响因子：
3.7
通讯作者：
Farr,RW
中科院分区：
文献类型：
--
作者：
Siddiqui,A;Gaynor,R;Srinivasan,A;Mapoles,J;Farr,RW
人B型肝炎病毒含有一个开放的阅读框，命名为X。我们研究了B型肝炎病毒X基因在调节转录控制元件中的反式激活功能。在HBV基因组中，X反式激活的主要靶标是增强子元件。此外，X蛋白刺激几种其他病毒启动子/增强子，包括人逆转录病毒的长末端重复序列（LTR）。详细研究的病毒序列之一是人类免疫缺陷病毒（HIV）LTR，它被X蛋白反式激活。使用HIV LTR的突变分析，我们表明U3区域内所含的NF-κ B序列参与了这种刺激活性。核连续分析支持X介导的反式激活发生在转录水平的概念。
Human hepatitis B virus contains an open reading frame designated X. We have investigated the trans-activating function of the hepatitis B virus X gene in regulating transcriptional control elements. In the HBV genome the major target for X trans-activation is the enhancer element. Further, the X protein stimulates several other viral promoters/enhancers including the long-terminal repeats (LTR) of human retroviruses. One of the viral sequences studied in detail is the human immunodeficiency viral (HIV) LTR which is trans-activated by the X protein. Using mutational analysis of the HIV LTR, we show that the NF-k B sequences contained within the U3 region are involved in this stimulatory activity. Nuclear run-on analyses support the notion that X-mediated trans-activation occurs at the level of transcription.