Direct attachment of suspension cells to PDA surface and its application in suspension-cell QCM biosensor
Direct attachment of suspension cells to PDA surface and its application in suspension-cell QCM biosensor
复制标题
悬浮细胞直接附着于PDA表面及其在悬浮细胞QCM生物传感器中的应用
DOI：
10.1016/j.snb.2020.128823
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发表时间：
2021-01-01
影响因子：
8.4
通讯作者：
Pei, Zhichao
中科院分区：
文献类型：
--
作者：
Li, Xueming;Song, QuanQuan;Pei, Zhichao
贻贝启发的聚多巴胺（PDA）已广泛应用于通过简单而通用的方法使各种材料表面功能化。几项研究已经证明了PDA表面对粘附细胞的粘附促进特性。然而，很少有报道与悬浮细胞。在这项研究中，悬浮细胞在PDA表面的附着性能进行了调查，我们发现，悬浮细胞可以直接附着到PDA涂层没有任何二次修饰。为了验证这一特性的潜力，基于PDA的悬浮细胞QCM生物传感器的制作。该生物传感器显示出高度的重复性和稳定性，以及低非特异性结合无关的蛋白质。不同范围的凝集素对三种不同类型的悬浮细胞的结合的实时和无标记的评价表明这些细胞表面上的糖基化的变化。此外，还对悬浮细胞表面的蛋白质-碳水化合物相互作用进行了动力学评价。本工作报道了一种通过简单有效的固定悬浮细胞来制备基于PDA的悬浮细胞QCM生物传感器的新策略，为悬浮细胞表面复杂分子识别的研究提供了一种简单的方法。
Mussel-inspired polydopamine (PDA) has been widely applied for functionalizing various material surfaces through a simple and versatile approach. Several studies have demonstrated the adhesion-promotion properties of the PDA surface for adherent cells. However, few reports are associated with suspension cells. In this study, the attachment property of suspension cells on the PDA surface was investigated for the first time, where we found that the suspension cells can be directly attached to the PDA coating without any secondary modifications. To exemplify the potential of this property, a PDA-based suspension-cell QCM biosensor was fabricated. The biosensor showed a high degree of repeatability and stability as well as low nonspecific binding to the irrelevant protein. The real-time and label-free evaluation of the binding of a diverse range of lectins on three different types of suspension cells indicated the variation of the glycosylation on these cell surfaces. Furthermore, the kinetic evaluation of the protein-carbohydrate interactions on the suspension cell surface was also performed. This work reports a new strategy for fabrication of PDA-based suspension-cell QCM biosensor via simple and efficient immobilization of suspension cells, which provides a simple way for the study of the complex molecular recognition on suspension cell surfaces.