Amh regulate female folliculogenesis and fertility in a dose-dependent manner through Amhr2 in Nile tilapia
Amh regulate female folliculogenesis and fertility in a dose-dependent manner through Amhr2 in Nile tilapia
复制标题
Amh 通过 Amhr2 在尼罗罗非鱼中以剂量依赖性方式调节雌性卵泡发生和生育能力
DOI：
10.1016/j.mce.2019.110593
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发表时间：
2020-01-01
影响因子：
4.1
通讯作者：
Wang, Deshou
中科院分区：
文献类型：
--
作者：
Liu, Xingyong;Xiao, Hesheng;Wang, Deshou
本研究通过免疫组织化学方法发现，Amh在罗非鱼初级生长卵泡的颗粒细胞中大量表达，Amhr 2在颗粒细胞、卵原细胞和I期卵母细胞中大量表达。此外，还发现Amh和Amhr 2在脑和垂体中表达。amh或amhr2的杂合突变导致初级生长卵泡增加和生育力下降，纯合突变导致卵巢肥大，初级卵泡显著增加，初级卵泡向卵黄发生卵泡的转变失败。两种突变体的脑中GnRH 3的表达、垂体中FSH和LH的表达以及血清E2浓度均显著降低。在两种突变体中均观察到滤泡细胞凋亡显着增加。然而，E2的管理未能挽救突变体的卵泡发生缺陷。我们的研究结果表明，AMH的作用，在罗非鱼的剂量依赖性的方式结合Amhr2。
In the present study, Amh was found to be abundantly expressed in the granulosa cells of the primary growth follicles, and Amhr2 in the granulosa cells, oogonia and phase I oocytes in tilapia by immunohistochemistry. In addition, Amh and Amhr2 were also found to be expressed in the brain and pituitary. Heterozygous mutation of either amh or amhr2 resulted in increased primary growth follicles and decreased fertility, and homozygous mutation resulted in hypertrophic ovaries with significantly increased primary follicles and failed transition from primary to vitellogenic follicles. Expression of gnrh3 in the brain, fsh and lh in the pituitary and serum E2 concentration were significantly decreased in both mutants. Significantly increased apoptosis of follicle cells was observed in both mutants. However, administration of E2 failed to rescue the folliculogenesis defects of the mutants. Our results suggested that Amh acts in a dose-dependent manner by binding Amhr2 in tilapia.