Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
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DOI：
10.1117/1.jbo.20.11.111206
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发表时间：
2015-11
影响因子：
3.5
通讯作者：
V. Kollárová;J. Čolláková;Zbyněk Doštál;P. Veselý;R. Chmelík
中科院分区：
文献类型：
--
作者：
V. Kollárová;J. Čolláková;Zbyněk Doštál;P. Veselý;R. Chmelík
抽象的。相干控制全息显微镜（CCHM）能够利用相干和非相干照明进行定量相位成像。低空间相干光会产生相干门控效应，这使得通过散射介质观察样品成为可能。本文利用CCHM对二维物体穿过静态散射层的成像进行了理论描述和数值模拟，重点关注定量相位成像质量。作者研究了以不同量的弹道光和漫射光为特征的强散射介质和弱散射介质。事实证明，对于静态、强散射层的情况也可以揭示相位信息。证明了成像过程的质量对空间光相干性的依赖性。理论计算和数值模拟得到了模型相物体以及在光学混浊乳液中处理的活癌细胞获得的实验数据的支持。
Abstract. A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion.