AN INVITRO MODEL OF IMMUNE COMPLEX-MEDIATED BASEMENT-MEMBRANE ZONE SEPARATION CAUSED BY PEMPHIGOID ANTIBODIES, LEUKOCYTES, AND COMPLEMENT
AN INVITRO MODEL OF IMMUNE COMPLEX-MEDIATED BASEMENT-MEMBRANE ZONE SEPARATION CAUSED BY PEMPHIGOID ANTIBODIES, LEUKOCYTES, AND COMPLEMENT
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DOI：
10.1111/1523-1747.ep12507222
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发表时间：
1982-01-01
影响因子：
6.5
通讯作者：
WHEELER, CE
中科院分区：
文献类型：
--
作者：
GAMMON, WR;MERRITT, CC;WHEELER, CE
描述了由类天疱疮抗体、血清补体和外周血白细胞引起的免疫复合物介导的基底膜区分离的体外模型。当新鲜冷冻的正常人皮肤的低温恒温器切片用含有补体激活抗基底膜区抗体的4种大疱性类天疱疮血清中的任一种处理并随后在37°C温育时。 C用正常人外周血白细胞和新鲜人血清，100%切片中白细胞附着于96%的基底膜区。在切片中，67% 出现基底膜区分离的局灶性区域，类似于早期类天疱疮病变中描述的真皮-表皮分离。在省略白细胞、类天疱疮抗体或新鲜人血清的对照切片中，白细胞附着和基底膜区分离明显减少。白细胞引起分离的证据得到了孵化期间对活白细胞的绝对要求、白细胞附着和分离之间的高度相关性以及表明附着于基底膜区域的白细胞被激活的实验的支持。这项研究提供了第一个体外证据，直接支持免疫复合物介导的炎症在大疱性类天疱疮的基底膜区分离和水疱的发病机制中的功能作用。
An in vitro model of immune complex-mediated basement membrane zone separation caused by pemphigoid antibodies, serum complement and peripheral blood leukocytes is described. When cryostat sections of fresh-frozen normal human skin were treated with either of 4 bullous pemiphigoid sera containing complement-activating anti-basement membrane zone antibodies and subsequently incubated at 37.degree. C with normal human peripheral blood leukocytes and fresh human serum, leukocytes attached to 96% of the basement membrane zone in 100% of sections. Of the sections, 67% developed focal areas of basement membrane zone separation resembling dermal-epidermal separation described in early pemphigoid lesions. In control sections in which either leukocytes, pemphigoid antibody or fresh human serum were omitted, significantly less leukocyte attachment and basement membrane zone separation occurred. Evidence that leukocytes caused separation was supported by an absolute requirement for viable leukocytes during incubation, a high correlation between leukocyte attachment and separation and experiments showing that leukocytes attached to the basement membrane zone were activated. This study provides the 1st in vitro evidence directly supporting a functional role for immune complex mediated inflammation in the pathogenesis of basement membrane zone separation and blisters in bullous pemphigoid.