Pure cultures for synthetic culture development: Next level municipal waste treatment for polyhydroxyalkanoates production
Pure cultures for synthetic culture development: Next level municipal waste treatment for polyhydroxyalkanoates production
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DOI：
10.1016/j.jenvman.2021.114337
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发表时间：
2021-12-28
影响因子：
8.7
通讯作者：
Cetecioglu, Zeynep
中科院分区：
文献类型：
--
作者：
Khatami, Kasra;Perez-Zabaleta, Mariel;Cetecioglu, Zeynep
聚羟基脂肪酸酯（PHAs）作为生物基塑料，有望从石油产品过渡到绿色和可持续的替代品。然而，它们的商业生产仍然受到高生产成本的阻碍。在这项研究中，我们评估了单一和共培养模式的合成培养细菌PHA生产。结果表明，挥发性脂肪酸（VFAs）来源于食物垃圾和初级污泥是廉价的碳源，以保持高产量的合成文化。从钩虫贪铜菌和洋葱伯克霍尔德菌中获得的PHA最大值分别为细胞干重的77.54 ± 5.67%（1.723 g/L）和54.9 ± 3.66%（1.088 g/L）。所获得的结果与使用糖底物的文献中的结果相当。相比之下，从共培养获得的PHA产量较低，范围在36-45 CDW%（0.39-0.48 g/L）之间。同时，生物聚合物中的3-羟基戊酸酯含量增加至21%。
Polyhydroxyalkanoates (PHAs), as bio-based plastics, promise a transition from petroleum products to green and sustainable alternatives. However, their commercial production is yet impeded by high production costs. In this study, we assessed synthetic culture in mono and co-culture modes for bacterial PHA production. It was demonstrated that volatile fatty acids (VFAs) derived from food waste and primary sludge are cheap carbon sources for maintaining high production yields in the synthetic cultures. The maximum obtained PHA was 77.54 +/- 5.67% of cell dried weight (CDW) (1.723 g/L) from Cupriavidus necator and 54.9 +/- 3.66% of CDW (1.088 g/L) from Burkholderia cepacia. The acquired results are comparable to those in literature using sugar substrates. Comparatively, lower PHA productions were obtained from the co-cultivations ranging between 36-45 CDW% (0.39-0.48 g/L). Meanwhile, the 3-hydroxyvalerate content in the biopolymers were increased up to 21%.