A GENERAL-METHOD FOR POLYETHYLENE-GLYCOL-INDUCED GENETIC-TRANSFORMATION OF BACTERIA AND YEAST
A GENERAL-METHOD FOR POLYETHYLENE-GLYCOL-INDUCED GENETIC-TRANSFORMATION OF BACTERIA AND YEAST
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DOI：
10.1016/0378-1119(83)90238-x
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发表时间：
1983-01-01
期刊：
影响因子：
3.5
通讯作者：
DOUGLAS, MG
中科院分区：
文献类型：
--
作者：
KLEBE, RJ;HARRISS, JV;DOUGLAS, MG
聚乙二醇 (PEG) 可以诱导细菌（大肠杆菌）和酵母（酿酒酵母）的遗传转化，而无需去除细胞壁。 PEG 介导的大肠杆菌转化技术简单，产生转化子的效率为 106-107 个转化子/.mu.g DNA。对 PEG 介导的大肠杆菌转化所涉及参数的详细分析揭示了 PEG 和标准 CaCl2 大肠杆菌转化方法之间的基本差异。 PEG 介导的酵母转化比现有的原生质体方法简单得多，并且效率相当。本文描述的 PEG 介导的遗传转化新方法可能被证明在多种生物体中进行遗传转化时具有通用性。
Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (E. coli) and yeast (S. cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 106-107 transformants/.mu.g DNA. Detailed analysis of the parameters involved in PEG-mediated transformation of E. coli reveals basic differences between the PEG and standard CaCl2 methods for transformation of E. coli. PEG-mediated transformation of yeast is far simpler than existing protoplast methods and is comparable in efficiency. The new methods described here for PEG-mediated genetic transformation may prove to be of general utility in performing genetic transformation in a wide variety of organisms.