Interleukin-6 production in human intestinal epithelial cells increases in association with the heat shock response
Interleukin-6 production in human intestinal epithelial cells increases in association with the heat shock response
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DOI：
10.1006/jsre.1998.5332
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发表时间：
1998-06-01
影响因子：
2.2
通讯作者：
Hasselgren, PO
中科院分区：
文献类型：
--
作者：
Parikh, AA;Moon, MR;Hasselgren, PO
背景在最近的研究中，IL-1 β刺激人肠上皮细胞中IL-6的产生。热休克反应影响某些细胞类型中炎症介质的产生。我们检验了热休克调节人肠上皮细胞中IL-1 β诱导的IL-6产生的假设。将培养的Caco-2细胞（一种人肠上皮细胞系）在43 ℃下暴露于热休克1小时，并在37 ℃下恢复1小时。收获细胞用于通过Western印迹分析热休克蛋白-70（HSP-70）的产生。在其他实验中，在热休克和恢复后加入IL-1 β（0.5 ng/ml）。24 h后用酶联免疫吸附法检测培养液中IL-6蛋白的表达，4 h后用竞争性逆转录聚合酶链反应检测培养液中IL-6 mRNA的表达。热休克导致HSP-70的产生并增强IL-1 β诱导的IL-6的产生。热休克反应与IL-6 mRNA水平升高有关。结果表明，IL-1 β诱导的人肠上皮细胞中IL-6的产生与热休克反应相关。肠上皮细胞中热休克增强IL-6产生的生物学作用仍有待确定。(C)北京：科学出版社.
Background. In recent studies, IL-1 beta stimulated the production of IL-6 in human enterocytes. The heat shock response influences the production of inflammatory mediators in certain cell types. We tested the hypothesis that heat shock regulates IL-1 beta-induced IL-6 production in human intestinal epithelial cells.Materials and methods. Cultured Caco-2 cells, a human intestinal epithelial cell Line, were exposed to thermal heat shock at 43 degrees C for 1 h and recovered at 37 degrees C for 1 h. Cells were harvested for analysis of heat shock protein-70 (HSP-70) production by Western blotting. In other experiments, IL-1 beta (0.5 ng/ml) was added following heat shock and recovery. IL-6 protein was measured in culture medium after 24 h by enzyme-linked immunosorbent assay and IL-6 messenger RNA (mRNA) levels were measured after 4 h by competitive reverse transcriptase polymerase chain reaction.Results. Heat shock resulted in the production of HSP-70 and potentiated IL-1 beta-induced IL-6 production. The response to heat shock was associated with increased IL-6 mRNA levels.Conclusions. The results suggest that IL-1 beta-induced IL-6 production in human enterocytes is increased in association with the heat shock response. The biological role of heat shock-potentiated IL-6 production in the enterocyte remains to be determined. (C) 1998 Academic Press.