Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells.
Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells.
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DOI：
10.1083/jcb.101.6.2036
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发表时间：
1985-12
期刊：
影响因子：
--
通讯作者：
Warren G
中科院分区：
文献类型：
--
作者：
Featherstone C;Griffiths G;Warren G
水疱性口炎病毒新合成的G蛋白在有丝分裂期间不被转运至培养的哺乳动物细胞的表面(Warren等人，1983，J.Cell Biol.97：1623-1628)。为了确定细胞内转运在何处受到抑制，我们检查了 G 蛋白的翻译后修饰，它是转运途径上特定区室的指标。有丝分裂细胞中的G蛋白只有含有七八个甘露糖残基的内切H敏感寡糖，但没有末端葡萄糖，并且没有被脂肪酰化。这些修饰表明仅由内质网 (ER) 的酶进行加工。定量免疫细胞化学被用作确认 G 蛋白从 ER 转运出受到抑制的独立方法。 ER 池中 G 蛋白的密度是经过类似处理的感染 G1 细胞的 2.5 倍。将受感染的有丝分裂细胞与放线菌酮一起孵育，放线菌酮可抑制蛋白质合成而不影响转运，但不会导致 ER 池中 G 蛋白的密度降低，这表明 G 蛋白不能被逐出 ER。这些结果表明细胞内转运在途径上第一个囊泡介导的步骤处或之前停止。
Newly synthesized G protein of vesicular stomatitis virus is not transported to the surface of cultured mammalian cells during mitosis (Warren et al., 1983, J. Cell Biol. 97:1623-1628). To determine where intracellular transport is inhibited, we have examined the post- translational modifications of G protein, which are indicators of specific compartments on the transport pathway. G protein in mitotic cells had only endo H-sensitive oligosaccharides containing seven or eight mannose residues, but no terminal glucose, and was not fatty acylated. These modifications were indicative of processing only by enzymes of the endoplasmic reticulum (ER). Quantitative immunocytochemistry was used as an independent method to confirm that transport of G protein out of the ER was inhibited. The density of G protein in the ER cisternae was 2.5 times greater than in infected G1 cells treated similarly. Incubation of infected mitotic cells with cycloheximide, which inhibits protein synthesis without affecting transport, did not result in a decrease in the density of G protein in the ER cisternae, demonstrating that G protein cannot be chased out of the ER. These results suggest that intracellular transport stops at or before the first vesicle-mediated step on the pathway.