Visualization and quantitation of spine deformity in zebrafish models of scoliosis by micro-computed tomography.
Visualization and quantitation of spine deformity in zebrafish models of scoliosis by micro-computed tomography.
复制标题
显微计算机断层扫描对斑马鱼脊柱侧凸模型脊柱畸形的可视化和定量研究。
DOI：
10.1016/j.xpro.2023.102739
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发表时间：
2023-12-15
期刊：
影响因子：
--
通讯作者：
Grimes DT
中科院分区：
文献类型：
--
作者：
Bearce EA;Ricamona BTB;Fisher KH;O'Hara-Smith JR;Grimes DT
斑马鱼（Danio rerio）越来越多地用于研究脊柱发育、生长，以及研究脊柱畸形（例如脊柱侧凸）的病因。在这里，我们提出了一种基于微计算机断层扫描的管道，用于可视化斑马鱼骨骼。我们描述了样品制备、成像、数据管理和处理的步骤。然后，我们使用开源软件对椎骨和脊柱形态进行详细分析。该协议对于科学家使用斑马鱼了解脊柱发育和疾病很有用。有关该协议的使用和执行的完整详细信息，请参阅 Bearce 等人。 （2022）。固定成年斑马鱼的微型计算机断层扫描指南 使用 3D 切片器手动分割骨骼结构的分步指南 进行脊柱形态测量的基本测量和科布角 出版商注：进行任何实验方案都需要遵守当地实验室安全和道德机构指南。斑马鱼（Danio rerio）越来越多地用于研究脊柱发育、生长，以及研究脊柱畸形（例如脊柱侧凸）的病因。在这里，我们提出了一种基于微计算机断层扫描的管道，用于可视化斑马鱼骨骼。我们描述了样品制备、成像、数据管理和处理的步骤。然后，我们使用开源软件对椎骨和脊柱形态进行详细分析。该协议对于科学家使用斑马鱼了解脊柱发育和疾病很有用。
Zebrafish (Danio rerio) are increasingly used to investigate spine development, growth, and for studying the etiology of spinal deformity, such as scoliosis. Here, we present a micro-computed tomography-based pipeline for visualizing the zebrafish skeleton. We describe steps for sample preparation, imaging, data management, and processing. We then detail analysis of vertebral and spine morphology using open-source software. This protocol will be useful for scientists using zebrafish to understand spine development and disease. For complete details on the use and execution of this protocol, please refer to Bearce et al. (2022). Guidance for micro-computed tomography scanning of fixed adult zebrafish Step-by-step guide for manual segmentation of skeletal structures with 3D Slicer Taking basic measurements and Cobb angles for spine morphometrics Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Zebrafish (Danio rerio) are increasingly used to investigate spine development, growth, and for studying the etiology of spinal deformity, such as scoliosis. Here, we present a micro-computed tomography-based pipeline for visualizing the zebrafish skeleton. We describe steps for sample preparation, imaging, data management, and processing. We then detail analysis of vertebral and spine morphology using open-source software. This protocol will be useful for scientists using zebrafish to understand spine development and disease.
特发性脊柱侧弯的斑马鱼模型将脑脊液流缺陷与脊柱曲率联系起来。
DOI：
10.1126/science.aaf6419
发表时间：
2016-06-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Grimes DT;Boswell CW;Morante NF;Henkelman RM;Burdine RD;Ciruna B
通讯作者：
Ciruna B
影响因子：
7.7
作者：
Bearce EA;Irons ZH;O'Hara-Smith JR;Kuhns CJ;Fisher SI;Crow WE;Grimes DT
通讯作者：
Grimes DT