Imbalance of Th17/Treg in Different Subtypes of Autoimmune Thyroid Diseases
Imbalance of Th17/Treg in Different Subtypes of Autoimmune Thyroid Diseases
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自身免疫性甲状腺疾病不同亚型中Th17/Treg失衡
DOI：
10.1159/000452541
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Zhang, Jin-an
中科院分区：
文献类型：
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作者：
Li, Cui;Yuan, Jianghong;Zhang, Jin-an
目的：阐明不同亚型自身免疫性甲状腺疾病（AITD）中 Th17/Treg 的失衡，包括格雷夫斯病（GD）、桥本甲状腺炎（HT）和格雷夫斯眼病（GO）。方法：47 例 AITD 患者（其中 16 例 GD、15 例 HT、16 例 GO）和 12 例健康对照者纳入本研究。采用流式细胞术和实时荧光定量PCR检测外周血单个核细胞(PBMC)中Th17、Treg细胞比例、Th17/Treg比值及其相关转录因子RORγt、Foxp3 mRNA表达量。 AITD 的 PBMC 中较高（P<0.05），特别是在 HT 亚组中（P<0.01）。 GD组PBMCs中CD4+Foxp3+T（Treg）细胞比例及其转录因子Foxp3 mRNA均显着降低（P<0.05）。此外，AITD组和GO亚组Th17/Treg比值升高（P<0.01）。 GO亚组中，临床活动评分（CAS）大于4.5的患者Th17比例高于CAS 3~4.5的患者（P<0.05）。结论：Th17淋巴细胞增多可能在HT和GO的发病中起更重要的作用，而Treg细胞减少可能在GD中发挥重要作用。
Aims: To clarify the imbalance of Th17/Treg in different subtypes of autoimmune thyroid diseases (AITDs) including Graves' disease(GD), Hashimoto's thyroiditis(HT) and Graves' ophthalmopathy (GO). Methods: 47 patients with AITD (including 16 GD, 15 HT, and 16 GO) and 12 healthy controls were enrolled in this study. The percentages of Th17 and Treg cells, the ratio of Th17/Treg, as well as their related transcription factors RORγt and Foxp3 mRNA in peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry and real-time quantitative PCR Results: Compared with those in control group, the percentage of CD4+IL-17+T cell(Th17) and the mRNA expression of its transcription factor RORγt were higher in PBMCs of AITDs (P<0.05), particularly in HT subgroup (P<0.01). The percentage of CD4+Foxp3+T (Treg) cells and its transcription factor Foxp3 mRNA were significantly decreased in PBMCs of GD (P<0.05). In addition, the ratio of Th17/Treg was elevated in AITD group and GO subgroup (P<0.01). In GO subgroup, the patients with clinical activity score (CAS) above 4.5 had higher percentages of Th17 than those with CAS ranging from 3 to 4.5 (P<0.05). Conclusion: Increased Th17 lymphocytes may play a more important role in the pathogenesis of HT and GO while decreased Treg may be greatly involved in GD.