Fibrinogen associated with subcellular platelet particles.
Fibrinogen associated with subcellular platelet particles.
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与亚细胞血小板颗粒相关的纤维蛋白原。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
N. Solum
中科院分区：
文献类型：
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作者：
H. Day;N. Solum
在EDTA缓冲介质中洗涤的血小板中，纤维蛋白原的亚细胞定位已被研究，特别是颗粒结合的纤维蛋白原。血小板匀浆经蔗糖密度梯度离心后，分离出9个不同密度的组分，并测定了它们的总蛋白、纤维蛋白原、β-葡萄糖醛酸酶和酸性对硝基苯基磷酸酶的相对含量。纤维蛋白原被发现在可溶相和颗粒馏分。相对于乳酸脱氢酶活性计算的纤维蛋白原的分布表明，颗粒结合的纤维蛋白原并不代表细胞质污染。结果进一步表明颗粒结合的纤维蛋白原与低密度颗粒的优先关联。β-葡萄糖醛酸酶和酸性磷酸酶也是如此。不仅存在纤维蛋白原，而且还存在两种溶酶体酶，在可溶性级分中含量高，表明该级分中的纤维蛋白原可能来源于破碎的颗粒。
The subcellular localization of fibrinogen in platelets washed in an EDTA-buffer medium has been studied with special reference to the granule-bound fibrinogen. After sucrose density gradient centrifugation of platelet homogenates nine fractions of different densities were separated and studied for their relative contents of total protein, fibrinogen, β-glucuronidase and acid p-nitrophenyl phosphatase. Fibrinogen was found both in the soluble phase and in the particulate fractions. The distribution of fibrinogen calculated relative to lactic dehydrogenase activity showed that granule-bound fibrinogen did not represent cytoplasmic contamination. The results further indicate a preferential association of granule-bound fibrinogen with low-density granules. The same was true for β-glucuronidase and acid phosphatase. Presence of not only fibrinogen, but also of the two lysosomal enzymes, in high amounts in the soluble fraction indicate that fibrinogen in this fraction may have been derived from disrupted granules.