Mibefradil-sensitive component involved in the plateau potential in submucosal interstitial cells of the murine proximal colon
Mibefradil-sensitive component involved in the plateau potential in submucosal interstitial cells of the murine proximal colon
复制标题
DOI：
10.1016/j.bbrc.2006.12.003
复制
发表时间：
2007-02-02
影响因子：
3.1
通讯作者：
Suzuki, Hikaru
中科院分区：
文献类型：
--
作者：
Hotta, Aya;Okada, Noriko;Suzuki, Hikaru
粘膜下Cajal间质细胞（ICCSM）产生由初始快速和随后的平台成分组成的平台电位。在小鼠近端结肠的ICCSM中检查了电压依赖性Ca 2+通道在平台电位中的可能参与。外部K+浓度（[K+]（o））的增加以双相方式改变了初始组分的上升速率，10.6或15.3 mM [K+]（o）增加，20.0 mM [K+]（o）减少。3 μ M米贝拉地尔或100 μ M Ni ~（2+）的应用显著降低了平台电位的上升速率，但0.3 μ M硝苯地平没有降低。米贝拉地尔对平台电位上升速率的抑制作用呈浓度依赖性，IC 50值为1.0 μ M。总之，平台电位的初始阶段部分是由于小鼠近端结肠ICCSM中T型Ca 2+通道的激活。(c)2006年爱思唯尔公司All rights reserved.
Submucosal interstitial cells of Cajal (ICCSM) produce plateau potentials comprised of initial fast and subsequent plateau components. The possible involvement of voltage-dependent Ca2+ channels in plateau potentials was examined in ICCSM of the murine proximal colon. Increases in external K+ concentration ([K+](o)) changed the rise rate of the initial component in a biphasic way, an increase in 10.6 or 15.3 mM [K+](o) and a decrease in 20.0 mM [K+](o). The rise rate of plateau potentials was significantly reduced by the application of 3 mu M mibefradil or 100 mu M Ni2+ but not by 0.3 mu M nifedipine. The inhibitory effect of mibefradil on the rise rate of plateau potentials was concentration-dependent with an IC50 value of 1.0 mu M. In conclusion, the initial phase of plateau potentials is partly due to the activation of T-type Ca2+ channel in ICCSM from the murine proximal colon. (c) 2006 Elsevier Inc. All rights reserved.