Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 2. Growth and function of transgenic rat insulinoma cells (betaG I/17).
Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 2. Growth and function of transgenic rat insulinoma cells (betaG I/17).
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具有胺化和氟化官能团混合物的组织培养表面。
DOI：
10.1163/156856203321478865
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Hoffman,AllanS
中科院分区：
文献类型：
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作者：
Bain,JamesR;Hoffman,AllanS
可移植细胞与合成聚合物的相互作用会影响生物杂交人工器官的功能。本研究探讨了βG I/17细胞在含有二胺基团(N_2)、三氟丙基(F_3)及其混合物的表面上生长和分泌人胰岛素的情况。培养在高F3和高氮气表面的细胞生长良好，生长迅速，每消耗一摩尔葡萄糖就会产生1.8摩尔乳酸，这与在许可的对照玻璃上生长的细胞非常相似。在一个摩尔比为33个N_2组：67个F_3组的混合面上，细胞的乳酸/葡萄糖比值较低，呈圆形，生长缓慢，能迅速形成突起的聚集体，类似于未处理的抑制对照聚苯乙烯的培养。F3含量较高的表面上的培养物分泌的胰岛素最多，在F3含量最高的表面上，培养物对促分泌剂的反应增强。当细胞生长在βG I/17早期培养的F3表面时，激素分泌大约增加50%。在重新接种之前，用高浓度的多克隆抗层粘连蛋白血清孵育条件表面，部分地消除了这种分泌功能的改善。含有三氟丙基的聚合物似乎是用于人工内分泌胰腺的有吸引力的候选者。含有层粘连蛋白的表面涂层在体外和体内都可能促进转基因胰岛素瘤细胞的功能。
Interactions of transplantable cells with synthetic polymers can influence the function of biohybrid artificial organs. This study explored growth and secretion of human insulin by βG I/17 cells cultured on surfaces bearing diamine groups (N2), trifluoropropyl groups (F3) and mixtures of the two. Cells cultured on high-F3 and high-N2 surfaces spread well, grew rapidly and produced >1.8 mol lactate per mol glucose consumed, closely resembling cells grown on the permissive control, glass. On one mixed surface, with a molar ratio of 33 N2 groups : 67 F3 groups, cells had a lower lactate/glucose ratio, adopted a rounded form, grew slowly and were quick to form emergent aggregates, similar to cultures on the inhibitory control, untreated polystyrene. Cultures on surfaces with higher F3 content secreted the most insulin and, in the case of the highest-F3 surface, showed improved responsiveness to secretagogues. Hormone secretion was roughly 50% greater when cells were grown on F3 surfaces conditioned by earlier cultures of βG I/17. Incubation of conditioned surfaces with high concentrations of a polyclonal anti-laminin serum prior to re-plating partially abolished this improvement in secretory function. Polymers bearing trifluoropropyl groups appear to be attractive candidates for use in the artificial endocrine pancreas. Surface coatings that include laminin might promote function of transgenic insulinoma cells in vitro and in vivo.