Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds
Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds
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DOI：
10.1016/j.electacta.2022.140240
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发表时间：
2022-03
影响因子：
6.6
通讯作者：
K. Ino;Keika Komatsu;Kaoru Hiramoto;Yoshinobu Utagawa;Yuji Nashimoto;H. Shiku
中科院分区：
文献类型：
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作者：
K. Ino;Keika Komatsu;Kaoru Hiramoto;Yoshinobu Utagawa;Yuji Nashimoto;H. Shiku
本研究描述了一种电化学发光（ECL）成像的细胞粘附在血管内皮细胞在水凝胶支架管形成过程中的方法。人脐静脉内皮细胞培养在基质胶涂层的氧化铟锡（ITO）电极，以促进小管生长。一旦形成这些管，将培养基更换为含有[Ru（bpy）3]2+和三丙胺的ECL溶液，然后使其经受1.2V vs Ag/AgCl（sat. KCl）以在ITO电极处产生ECL信号。这允许细胞粘附的ECL成像，因为这些ECL信号在以强细胞-细胞和细胞-底物粘附为代表的区域中被阻断。图像显示，在管形成过程中有两种类型的细胞粘附。据我们所知，这是第一次报告的ECL成像细胞之间的粘附和细胞和基板之间的管形成过程中的水凝胶支架。我们相信，这种ECL成像技术将是有用的评估器官的芯片和药物筛选靶向细胞和细胞基质相互作用的未来。
This study describes a method for electrochemiluminescence (ECL) imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds. Human umbilical vein endothelial cells were cultured on a Matrigel-coated indium tin oxide (ITO) electrode to facilitate tubule growth. Once these tubes were formed, the culture medium was changed to an ECL solution containing [Ru(bpy)3]2+and tripropylamine and then subjected to 1.2 V vs Ag/AgCl (sat. KCl) to produce ECL signals at the ITO electrode. This allowed for ECL imaging of cellular adhesion because these ECL signals were blocked in areas typified by strong cell–cell and cell–substrate adhesion. The imaging showed that there were two types of cellular adhesion during tube formation. To the best of our knowledge, this is the first report on ECL imaging for adhesion between cells and between cells and substrates during tube formation on hydrogel scaffolds. We believe that this ECL imaging technique will be useful for the evaluation of organs-on-a-chip and for drug screening targeting cell–cell and cell–substrate interactions in the future.