Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance.
Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance.
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禽致病性大肠杆菌铁调节基因ireA参与粘附和抗应激
DOI：
10.1186/s12917-016-0800-y
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发表时间：
2016-08-17
影响因子：
2.6
通讯作者：
Tang F
中科院分区：
文献类型：
--
作者：
Li Y;Dai J;Zhuge X;Wang H;Hu L;Ren J;Chen L;Li D;Tang F
禽致病性大肠杆菌（APEC）是引起禽大肠杆菌病的主要病原菌之一，在世界范围内给养禽业带来了巨大的经济和福利损失。对禽致病性E.大肠杆菌菌株没有很好的定义。本文对禽致病性大肠杆菌ireA基因编码的外膜蛋白的功能进行了研究。大肠杆菌DE 205 B。ireA基因在140株大肠杆菌中的分布率为32.9%（46/140）.大肠埃希菌ECOR类群以B2（58.8%，10/17）和D（55.9%，19/34）为主。APEC菌株DE 205 B在高铁M9培养基中的ireA基因表达量是低铁M9培养基中的1.8倍（P < 0.05）。构建了ireA缺失突变株和互补株。与野生型菌株DE 205 B相比，IreA缺失突变株的大多数铁吸收基因的表达均显著上调（P <0. 05）。IreA缺失突变体对DF-1细胞的粘附能力显著降低。在碱性、高渗透压和低温（4 °C）条件下，ireA缺失突变株的存活率分别比野生型降低21.17%（P <0.01）、25.42%（P < 0.05）和70.0%（P < 0.01）。结果表明，铁调控基因ireA编码的蛋白在禽致病性大肠杆菌中具有粘附和抗应激作用。杆菌本文的在线版本（doi：10.1186/s12917-016-0800-y）包含补充材料，可供授权用户使用。
Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis, which results in economic and welfare costs in the poultry industry worldwide. The pathogenesis of avian pathogenic E. coli strains is not well defined. Here, the function of an outer membrane protein encoded by the ireA gene of avian pathogenic E. coli strain DE205B was investigated. The ireA gene was distributed in 32.9 % (46/140) of tested E. coli strains, with high percentages in the phylogenetic ECOR groups B2 (58.8 %, 10/17) and D (55.9 %, 19/34). The gene expression level of ireA of APEC strain DE205B in high Fe M9 media was 1.8 times higher (P < 0.05) than that in low Fe M9 media. An ireA deletion mutant and complementary strain were constructed. Compared with the wild-type strain DE205B, the expression of most ferric uptake genes in the ireA deletion mutant were significantly upregulated (P < 0.05). The adhesion ability of the ireA deletion mutant to DF-1 cells was significantly decreased. The survival rate of ireA deletion mutant was reduced 21.17 % (P < 0.01), 25.42 (P < 0.05) and 70.0 % (P < 0.01) under alkali, high osmolarity, and low temperature (4 °C) conditions, respectively, compared with the wild-type strain. The results suggested that the protein encoded by the iron-regulated gene ireA has roles in adhesion and stress resistance in avian pathogenic E. coli. The online version of this article (doi:10.1186/s12917-016-0800-y) contains supplementary material, which is available to authorized users.