Distribution of [35S]dATP alpha S binding sites in the adult rat neuraxis.
Distribution of [35S]dATP alpha S binding sites in the adult rat neuraxis.
复制标题
[35S]dATP αS 结合位点在成年大鼠神经轴中的分布。
作者：
J. Simon;T. E. Webb;E. Barnard
[35S]2‘-脱氧腺苷5’-O-(1-硫代)三磷酸([35S]dATPαS，KD：9+/-2 NM；Bmax：39+/-8pmol/mg蛋白)在成年大鼠脑膜上存在高度丰富的、可饱和的和特异的结合部位，其特征与预期的P2Y1受体一致。用体外放射自显影技术研究这些结合部位在脑和脊髓中的解剖分布。[35S]dATPαS结合位点广泛分布于脑和脊髓。它们可以被大量(100微米)的2-甲硫基三磷酸腺苷(2MeS-ATP)取代，但不能被尿苷-5‘-三磷酸(UTP)或α，β-亚甲基三磷酸腺苷(α，β-meATP)取代。在皮质区域内，标记具有相同的中等密度。然而，嗅球、皮质下端脑、海马复合体、丘脑区和中脑内的离散结构和核显示出不同的密度。在脊髓内，灰质的标记密度比FUNICULI高。本研究阐明了P2Y1及其密切相关受体在大鼠中枢神经系统内的解剖分布，并进一步证实了这些受体在神经轴中丰富且广泛的分布。
Highly abundant, saturable and specific binding sites for [35S]2'-deoxyadenosine 5'-O-(1-thio) triphosphate ([35S]dATP alpha S, Kd: 9 +/- 2 nM; Bmax: 39 +/- 8 pmol/mg protein) are present in adult rat brain membranes and have characteristics consistent with those expected for a P2Y1 receptor. The anatomical distribution of these binding sites in the brain and spinal cord was examined using in vitro autoradiography. The [35S]dATP alpha S binding sites showed a widespread distribution throughout the brain and spinal cord. They could be displaced by a large excess (100 microM) of 2-methylthioATP (2MeS-ATP) but not by uridine-5'-triphosphate (UTP) or alpha,beta-methyleneATP (alpha,beta-meATP). Within the cortical regions labelling was of equal medium density. However, discrete structures and nuclei within the olfactory bulb, subcortical telencephalon, hippocampal complex, thalamic regions and mesencephalon displayed a variety of densities. Within the spinal cord, gray matter was labelled at a greater density than the funiculi. The present study clarifies the anatomical distribution of P2Y1 and closely related receptors within the central nervous system of rat and extends the evidence that those receptors are abundant and widely distributed within the neuraxis.