Effect of chloride ions in Tris buffer solution on bioactive glass apatite mineralization
Effect of chloride ions in Tris buffer solution on bioactive glass apatite mineralization
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DOI：
10.1111/ijag.12288
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发表时间：
2017-12-01
影响因子：
2.1
通讯作者：
Brauer, Delia S.
中科院分区：
文献类型：
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作者：
Kirste, Gloria;Brandt-Slowik, Juliane;Brauer, Delia S.
研究了Bioglass 45S5在两种不同的三羟甲基氨基甲烷(Tris)缓冲溶液中的降解和磷灰石沉淀。在常规方法中，用盐酸(Tris-HCl缓冲液)调节pH至7.4。在另一种方法中，使用了醋酸，从而得到了一种几乎不含氯离子的Tris缓冲溶液(Tris-HAc)。EDX结果表明，Tris-HCl缓冲溶液中的氯离子被引入到浸泡实验中形成的磷灰石中，形成(部分)取代的氯磷灰石。在Tris-HAc中没有检测到沉淀的磷灰石中的氯离子。虽然在所研究的时间点(6小时至7天)内，磷灰石的形成速度没有显著差异，但氯离子的存在可能会影响磷灰石形成的非常早期的阶段。因此，在研究生物活性玻璃的早期时间点释放动力学或磷灰石沉淀时，使用不包含参与磷灰石形成的高浓度离子的改良Tris缓冲液可能是有利的。
Degradation and apatite precipitation of Bioglass 45S5 were characterized in two different tris(hydroxymethyl)aminomethane (Tris) buffer solutions. In the conventional one, pH was adjusted to 7.4 using hydrochloric acid (Tris-HCl buffer). In the other one, acetic acid was used instead, thereby obtaining a Tris buffer solution, which was virtually free of chloride ions (Tris-HAc). EDX results showed that chloride ions present in Tris-HCl buffer solution were incorporated into the apatite formed during immersion experiments, forming a (partially) substituted chlorapatite. No chloride ions were detected in apatite precipitated in Tris-HAc. Although no significant differences in the rate of apatite formation were observed during the time points studied here (6 hours to 7 days), the presence of chloride ions may affect very early stages of apatite formation. It may therefore be advantageous to use modified Tris buffer solutions, which do not contain high concentrations of ions involved in apatite formation, when studying early time point release kinetics or apatite precipitation of bioactive glasses.