SELEX and SHAPE reveal that sequence motifs and an extended hairpin in the 5' portion of Turnip crinkle virus satellite RNA C mediate fitness in plants.
SELEX and SHAPE reveal that sequence motifs and an extended hairpin in the 5' portion of Turnip crinkle virus satellite RNA C mediate fitness in plants.
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SELEX 和 SHAPE 揭示了芜菁皱纹病毒卫星 RNA C 5 部分的序列基序和延伸发夹介导植物的适应性。
DOI：
10.1016/j.virol.2018.05.010
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Simon,Anne
中科院分区：
文献类型：
--
作者：
Bayne,CharlieF;Widawski,MaxE;Gao,Feng;Masab,MohammedH;Chattopadhyay,Maitreyi;Murawski,AllisonM;Sansevere,RobertM;Lerner,BryanD;Castillo,RinaldysJ;Griesman,Trevor;Fu,Jiantao;Hibben,JenniferK;Garcia-Perez,AlmaD;Simon,Anne
非编码RNA使用它们的序列和/或结构来介导功能。芜菁皱缩病毒（Turnipcrinklevirus，TCV）非编码卫星RNA C（satC）的5'端（166 nt）含有一个中心区，中心区有两个茎环（H6和H7）和一个大的连接发夹（H2）。我们现在报道，评估H2、H6和H7中的序列/结构要求的体内功能选择（SELEX）实验显示，H6环序列基序以非随机速率回收，并且只有一些残基被提出与5'中心区域内的可接近互补序列进行碱基配对。使得沿着下部茎和H2区，存在一个构成整个5'区的延伸发夹。由于这些SELEX获奖者是高度适合的，这些特征促进了植物中与TCV相关的satRNA扩增。
Noncoding RNAs use their sequence and/or structure to mediate function(s). The 5’ portion (166 nt) of the 356-nt noncoding satellite RNA C (satC) ofTurnip crinkle virus(TCV) was previously modeled to contain a central region with two stem-loops (H6 and H7) and a large connecting hairpin (H2). We now report thatin vivofunctional selection (SELEX) experiments assessing sequence/structure requirements in H2, H6, and H7 reveal that H6 loop sequence motifs were recovered at nonrandom rates and only some residues are proposed to base-pair with accessible complementary sequences within the 5’ central region.In vitroSHAPE of SELEX winners indicates that the central region is heavily base-paired, such that along with the lower stem and H2 region, one extensive hairpin exists composing the entire 5’ region. As these SELEX winners are highly fit, these characteristics facilitate satRNA amplification in association with TCV in plants.