BIDIRECTIONAL EFFECTS OF GABAERGIC AGONISTS AND ANTAGONISTS ON MAINTENANCE OF VOLUNTARY ETHANOL INTAKE IN RATS
BIDIRECTIONAL EFFECTS OF GABAERGIC AGONISTS AND ANTAGONISTS ON MAINTENANCE OF VOLUNTARY ETHANOL INTAKE IN RATS
复制标题
DOI：
10.1016/0091-3057(93)90338-t
复制
发表时间：
1993-09-01
影响因子：
3.6
通讯作者：
AMIT, Z
中科院分区：
文献类型：
--
作者：
BOYLE, AE;SEGAL, R;AMIT, Z
研究了 THIP（GABA(A) 激动剂）和印防己毒素（GABA 拮抗剂）对维持自愿乙醇摄入的影响。 33 只雄性 Long-Evans 大鼠最初接受筛选程序，其中按隔日时间表自由选择提供逐渐增加浓度的乙醇（从 2% 至 9%）和水。筛选程序之后，将大鼠暴露于五次浓度为 9% 的乙醇，构成基线期，并暴露于五次额外的乙醇，在此期间确定 GABA 能操作的效果（测试期）。在测试期间，动物接受IP注射16mg/kg的THIP、2mg/kg的印防己毒素或盐水。结果表明，不同的 GABA 操作会对乙醇的消耗产生双向影响。更具体地说，与基线测量相比，GABA(A) 激动剂 THIP 增加了乙醇摄入量，而 GABA 拮抗剂印防己毒素减少了乙醇摄入量。同样，THIP 的施用增加了对乙醇的偏好。相比之下，施用印防己毒素后，对乙醇的偏好相对于水有所降低。这些 GABA 能操作的效果似乎是针对乙醇的，因为总液体摄入量不受任何一种药物（即 THIP 或印防己毒素）给药的影响。鉴于文献表明 THIP 和印防己毒素在 GABA(A) 氯离子载体受体复合物内的不同位点具有活性，目前的研究结果表明 GABA(A) 受体可能在调节乙醇的自愿摄入中发挥作用。
The effects of THIP (GABA(A) agonist) and picrotoxin (GABA antagonist) on the maintenance of voluntary ethanol ingestion were examined. Thirty-three male Long-Evans rats were initially exposed to a screening procedure in which increasing concentrations of ethanol (from 2% to 9%) were presented in a free choice with water, on an alternate day schedule. Following the screening procedure, the rats were exposed to five ethanol presentations at a concentration of 9%, which constituted the baseline period, and five additional ethanol presentations during which the effects of the GABAergic manipulations were determined (test period). During the test period, the animals received IP injections of either 16 mg/kg of THIP, 2 mg/kg of picrotoxin or saline. The results suggested that the differential GABA manipulations resulted in bidirectional effects on the consumption of ethanol. More specifically, the GABA(A) agonist THIP increased the intake of ethanol as compared to baseline measures, while the GABA antagonist picrotoxin decreased ethanol intake. Similarly, the administration of THIP increased ethanol preference. In contrast, preference for ethanol over water was decreased following the administration of picrotoxin. It appears that the effects of these GABAergic manipulations are specific to ethanol, since total fluid intake was not influenced by the administration of either drug (i.e., THIP or picrotoxin). In light of the literature suggesting that THIP and picrotoxin are active at different sites within the GABA(A) chloride-ionophore receptor complex, the present findings would suggest that the GABA(A) receptor may play a role in regulating the voluntary intake of ethanol.