Alcoholics show reduced telomere length in the oesophagus
Alcoholics show reduced telomere length in the oesophagus
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DOI：
10.1002/path.2817
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发表时间：
2011-02-01
影响因子：
7.3
通讯作者：
Takubo, Kaiyo
中科院分区：
文献类型：
--
作者：
Aida, Junko;Yokoyama, Akira;Takubo, Kaiyo
端粒是位于染色体末端的重复的富含G的DNA序列。端粒功能障碍引起的染色体和基因组不稳定性在肿瘤的发生中起着重要作用。为了研究酗酒者食道上皮细胞的端粒缩短，我们使用Q-FISH和我们的原始软件TELO测量了基底细胞和基底旁细胞的端粒长度，并与非酗酒者的端粒长度进行了比较，同时还评估了组织炎症。与年龄匹配的正常对照组相比，酗酒者基底细胞中的端粒显著缩短。无论是酗酒者还是非酗酒者，慢性炎症的显著组织学发现都不明显。我们发现酗酒者食道上皮中的端粒比非酗酒者短，这表明端粒缩短可能与酗酒者鳞状细胞癌的频繁发生有关。进一步的研究将有必要阐明酒精中毒患者食道上皮中端粒长度每年大量丢失的原因，这些端粒长度变化很快或端粒酶活性较低。版权所有(C)2010年大不列颠和爱尔兰病理学会。作者：John Wiley&Sons，Ltd.
Telomeres are repetitive G-rich DNA sequences located at the ends of chromosomes. Chromosomal and genomic instability due to telomere dysfunction plays an important role in carcinogenesis. To study telomere shortening in the oesophageal epithelium of alcoholics, we measured the telomere lengths of basal and parabasal cells in comparison with those of non-alcoholics using Q-FISH and our original software, Tissue Telo, and also assessed histological inflammation. Telomeres in basal cells were significantly shorter in alcoholics than in age-matched normal controls. Prominent histological findings of chronic inflammation were not evident in either alcoholics or non-alcoholics. Our finding that telomeres in the oesophageal epithelium are shorter in alcoholics than in non-alcoholics indicates that telomere shortening may be associated with the frequent occurrence of squamous cell carcinoma in alcoholics. Further studies to clarify the reason for the large annual loss of telomere length with rapid turnover or lower telomerase activity in the oesophageal epithelium of alcoholics will be necessary. Copyright (C) 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.