A comparative genomics study of 23 Aspergillus species from section Flavi
A comparative genomics study of 23 Aspergillus species from section Flavi
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DOI：
10.1038/s41467-019-14051-y
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发表时间：
2020-02-27
影响因子：
16.6
通讯作者：
Andersen, Mikael R.
中科院分区：
文献类型：
--
作者：
Kjaerbolling, Inge;Vesth, Tammi;Andersen, Mikael R.
黄曲霉部分包括有害和有益的曲霉菌种，如用于食品发酵和酶生产的米曲霉，以及食品搅拌器和霉菌毒素生产者黄曲霉。在这里，我们测序了19个跨越黄组的基因组，并比较了包括23个黄组种在内的31个真菌基因组。我们重新评估了它们的系统发育关系，发现米曲霉最近的亲缘关系不是黄曲霉，而是微小核盘菌或黄曲霉，并鉴定了高度的基因组多样性，特别是在亚端粒区域。我们预测在Flavi组中有丰富的CAZymes(每个物种598个)和多产的次生代谢物基因簇(每个物种73个)。然而，观察到的表型(生长特征、多糖降解)并不一定与根据预测的CAZyme含量做出的推断相关。我们的工作，包括基因组分析、表型分析和次生代谢物的鉴定，突出了Flavi组的遗传和代谢多样性。
Section Flavi encompasses both harmful and beneficial Aspergillus species, such as Aspergillus oryzae, used in food fermentation and enzyme production, and Aspergillus flavus, food spoiler and mycotoxin producer. Here, we sequence 19 genomes spanning section Flavi and compare 31 fungal genomes including 23 Flavi species. We reassess their phylogenetic relationships and show that the closest relative of A. oryzae is not A. flavus, but A. minisclerotigenes or A. aflatoxiformans and identify high genome diversity, especially in sub-telomeric regions. We predict abundant CAZymes (598 per species) and prolific secondary metabolite gene clusters (73 per species) in section Flavi. However, the observed phenotypes (growth characteristics, polysaccharide degradation) do not necessarily correlate with inferences made from the predicted CAZyme content. Our work, including genomic analyses, phenotypic assays, and identification of secondary metabolites, highlights the genetic and metabolic diversity within section Flavi.