Infection pattern and transmission potential of chikungunya virus in two New World laboratory-adapted Aedes aegypti strains
Infection pattern and transmission potential of chikungunya virus in two New World laboratory-adapted Aedes aegypti strains
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DOI：
10.1038/srep24729
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发表时间：
2016-04-22
影响因子：
4.6
通讯作者：
Franz, Alexander W. E.
中科院分区：
文献类型：
--
作者：
Dong, Shengzhang;Kantor, Asher M.;Franz, Alexander W. E.
基孔肯雅病毒(Chikungenya Virus，CHIKV)是由埃及伊蚊和埃及伊蚊传播给人类的一种新型蚊媒病毒。白纹伊蚊。我们描述了CHIKV在两个新大陆Ae的感染模式。埃及伊蚊、HWE和ORL。这两种蚊子对病毒都很敏感，但在中肠和唾液腺中表现出不同的感染模式。尽管获取血餐显示中肠组织中有中等水平的细胞凋亡，但在中肠感染期间没有检测到明显的CHIKV诱导的细胞凋亡。对细胞凋亡相关基因表达的分析表明，CHIKV感染抑制而不是促进蚊子中肠的细胞凋亡。在这两种蚊子中，病毒在口腔感染后两天内出现在唾液中。HWE和ORL蚊子没有唾液腺感染屏障；然而，只有60%(HWE)至65%(ORL)的雌性蚊子在口服后一周在唾液中释放病毒，这表明唾液腺存在逃逸屏障。CHIKV在HWE和ORL蚊子的唾液腺中诱导了细胞凋亡反应，表明该病毒在其自然媒介中引起了病理。
Chikungunya virus (CHIKV) is an emerging mosquito-borne virus belonging to the Togaviridae, which is transmitted to humans by Aedes aegypti and Ae. albopictus. We describe the infection pattern of CHIKV in two New World Ae. aegypti strains, HWE and ORL. Both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. Even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious additional CHIKV-induced apoptosis detectable during midgut infection. Analysis of expression of apoptosis-related genes suggested that CHIKV infection dampens rather than promotes apoptosis in the mosquito midgut. In both mosquito strains, the virus was present in saliva within two days post-oral infection. HWE and ORL mosquitoes exhibited no salivary gland infection barrier; however, only 60% (HWE) to 65% (ORL) of the females had released the virus in their saliva at one week post-oral acquisition, suggesting a salivary gland escape barrier. CHIKV induced an apoptotic response in salivary glands of HWE and ORL mosquitoes, demonstrating that the virus caused pathology in its natural vector.