Splicing of the Drosophila P element ORF2-ORF3 intron is inhibited in a human cell extract.
Splicing of the Drosophila P element ORF2-ORF3 intron is inhibited in a human cell extract.
复制标题
果蝇 P 元件 ORF2-ORF3 内含子的剪接在人细胞提取物中受到抑制。
DOI：
10.1016/0925-4773(91)90042-5
复制
发表时间：
1991
影响因子：
2.6
通讯作者：
Laski,FA
中科院分区：
文献类型：
--
作者：
Tseng,JC;Zollman,S;Chain,AC;Laski,FA
P element transposition in Drosophila melanogaster is regulated by germline-specific splicing of the P element ORF2-ORF3 intron. This regulation has been shown to depend on a cis-acting sequence located in the exon 12–31 bases from the 5′ splice site. Mutations within this sequence disrupt the regulation and result in splicing of the ORF2-ORF3 intron in all tissues, indicating that the sequence is required to inhibit splicing of this intron in the soma. We now show that a trans-acting factor in a human (HeLa) cell extract can inhibit splicing of the intron, suggesting that this regulatory mechanism is conserved from flies to humans.
