Comparative study of the effects of short- and long-term ethanol treatment and alcohol withdrawal on phospholipid biosynthesis in rat hepatocytes
Comparative study of the effects of short- and long-term ethanol treatment and alcohol withdrawal on phospholipid biosynthesis in rat hepatocytes
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DOI：
10.1016/s1096-4959(02)00006-4
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发表时间：
2002-03-01
影响因子：
2.2
通讯作者：
Marco, C
中科院分区：
文献类型：
--
作者：
Carrasco, MP;Jiménez-López, JM;Marco, C
本研究描述了短期和长期乙醇处理和撤药对从大鼠分离的肝细胞中磷脂磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）生物合成的影响，使用同位素标记的胆碱和乙醇胺作为外源性前体。我们的研究结果表明，短期的乙醇消耗增加了外源性极性碱基的PC和PE的掺入，而长期的乙醇管理引起的差异PC和PE生物合成的影响，通过胞苷二磷酸衍生物（CDP衍生物），减少PC的合成和增加PE的生物合成。我们认为，乙醇处理后的PE的生物合成增加的结果从脂肪生成底物的变化产生的乙醇代谢的结果，而PC生物合成的特异性抑制似乎是参与CDP-胆碱途径的酶的改变的结果。关于乙醇对PE甲基化产生PC的影响，我们的研究结果表明，乙醇在短期内激活这一途径，以及慢性乙醇治疗。乙醇戒断使PC和PE途径的活性恢复到对照水平。在生物合成的主要磷脂，PC和PE，在这项研究中表现出的改变可能是一个巨大的生理利益，在确定酒精中毒的病理。(C)2002年爱思唯尔科技有限公司All rights reserved.
This study describes the effects of short- and long-term ethanol treatment and withdrawal on the biosynthesis of the phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in hepatocytes isolated from rats, using isotopically labelled choline and ethanolamine as exogenous precursors. Our results demonstrate that short-term ethanol consumption increases the incorporation of exogenous polar bases into PC and PE, whereas long-term ethanol administration provokes a differential effect in both PC and PE biosynthesis via cytidine diphosphate derivatives (CDP-derivatives), decreasing PC synthesis and increasing the biosynthesis of PE. We suggest that the increased biosynthesis of PE after ethanol treatment results from changes in lipogenic substrates produced as a consequence of ethanol metabolism, whilst the specific inhibition of PC biosynthesis seems to be a consequence of alterations of enzymes involved in the CDP-choline pathway. With regard to the influence of ethanol on PE methylation to give PC, our results demonstrate that ethanol activates this pathway in short-term, as well as chronic ethanol treatment. Ethanol withdrawal returns the activity of the PC and PE pathways to control levels. The alterations in the biosynthesis of the main phospholipids, PC and PE, demonstrated in this study could be of a great physiological interest in determining the pathology of alcoholism. (C) 2002 Elsevier Science Inc. All rights reserved.