A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development
A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development
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DOI：
10.1104/pp.107.095737
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发表时间：
2007-05-01
期刊：
影响因子：
7.4
通讯作者：
Zhou, Dao-Xiu
中科院分区：
文献类型：
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作者：
Dai, Mingqiu;Hu, Yongfeng;Zhou, Dao-Xiu
YABBY和WOX基因在侧生器官形成和分生组织功能中起重要作用。本文报道了YAB 3和WOX 3在水稻叶片发育中的功能关系。水稻YAB 3与玉米（Zea mays）ZmYAB 14和拟南芥（Arabidopsis thaliana）丝状花（FIL）密切相关，而水稻WOX 3与玉米窄鞘1（NS 1）和NS 2以及拟南芥PRESSED FLOWER（PRS）高度保守。原位杂交实验表明，这两个基因的表达被排除在茎尖分生组织，但转录本检测到叶原基，幼叶，生殖器官没有任何极性分布。通过过量表达和RNA干扰（RNAi）技术研究了这两个基因在转基因水稻中的功能。YAB 3 RNAi诱导了叶片扭曲和打结，缺乏叶舌和叶耳等特化结构，而在YAB 3过表达植株中没有观察到表型变化，表明水稻YAB 3可能是叶细胞生长和分化所必需的。WOX 3的过表达抑制了YAB 3，并显示出YAB 3 RNAi表型。在YAB 3 RNAi或WOX 3过表达植物的叶片中异位诱导I类KNOTTED-LIKE HOMEOBOX（KNOX）基因的表达。从诱导WOX 3表达和DNA-蛋白质相互作用分析的数据表明，WOX 3作为YAB 3的转录抑制因子。这些数据揭示了水稻叶片发育所需的YAB 3，WOX 3和KNOX基因的调控网络。
YABBY and WUSCHEL-LIKE HOMEOBOX (WOX) genes have been shown to play important roles in lateral organ formation and meristem function. Here, we report the characterization of functional relationship between rice (Oryza sativa) YAB3 and WOX3 in rice leaf development. Rice YAB3 is closely related to maize (Zea mays) ZmYAB14 and Arabidopsis (Arabidopsis thaliana) FILAMENTOUS FLOWER (FIL), whereas rice WOX3 is highly conserved with maize narrow sheath1 (NS1) and NS2 and Arabidopsis PRESSED FLOWER (PRS). In situ hybridization experiments revealed that the expression of both genes was excluded from the shoot apical meristem, but the transcripts were detected in leaf primordia, young leaves, and reproductive organs without any polar distribution. The function of the two genes was studied by both overexpression and RNA interference (RNAi) in transgenic rice. YAB3 RNAi induced twisted and knotted leaves lacking specialized structures such as ligule and auricles, while no phenotypic change was observed in YAB3 overexpression plants, suggesting that rice YAB3 may be required for leaf cell growth and differentiation. Overexpression of WOX3 repressed YAB3 and showed a YAB3 RNAi phenotype. The expression of class I KNOTTED-LIKE HOMEOBOX (KNOX) genes was ectopically induced in leaves of YAB3 RNAi or WOX3 overexpression plants. Data from inducible WOX3 expression and DNA-protein interaction assays suggested that WOX3 acted as a transcriptional repressor of YAB3. These data reveal a regulatory network involving YAB3, WOX3, and KNOX genes required for rice leaf development.