The active E rosette test: correlation with delayed cutaneous hypersensitivity.
The active E rosette test: correlation with delayed cutaneous hypersensitivity.
复制标题
主动 E 玫瑰花结试验：与迟发性皮肤超敏反应的相关性。
DOI：
10.4049/jimmunol.116.4.1110
复制
发表时间：
1976
影响因子：
4.4
通讯作者：
R. H. Bilman
中科院分区：
文献类型：
--
作者：
P. Felsburg;R. Edelman;R. H. Bilman
在用微生物抗原进行皮肤测试后，在正常人中测量了外周血T淋巴细胞的活跃亚群，其特征是与绵羊红细胞（A-RFC）快速（5分钟）形成玫瑰花结。所有迟发性皮肤超敏反应（DCH）阳性的个体A- rfc显著升高，而皮肤试验无反应者A- rfc无显著升高。在总T细胞群体中没有发生类似的一致变化，其特征是与绵羊红细胞形成更长（60分钟）的玫瑰花结，或者在B细胞中，通过表面免疫球蛋白的免疫荧光测量。A-RFC反应在时间上与DCH反应平行，但在强度上不同。这些结果为生物学上独特的T细胞亚群提供了体内证据，并将注意力集中在a - rfc作为免疫活性细胞上。
An active subpopulation of peripheral blood T lymphocytes, characterized by rapid (5-min) rosette formation with sheep erythrocytes (A-RFC), was measured in normal individuals after they were skin tested with microbial antigens. A significant rise in A-RFC occurred in all individuals who developed positive delayed cutaneous hypersensitivity (DCH) reactions, whereas skin test nonresponders showed no significant rise in A-RFC. No similar consistent changes occurred in populations of total T cells, characterized by longer (60-min) rosette formation with sheep erythrocytes, or in B cells, measured by immunofluorescence of surface immunoglobulin. The A-RFC response paralleled the DCH response in timing, but not in intensity. These results provide in vivo evidence for a biologically distinct T cell subpopulation, and focus attention on the A-RFC as immunologically active cells.