Five Postmortem Case Reports with Qualitative Analysis of Cyclopropylfentanyl by LC-MS-MS
Five Postmortem Case Reports with Qualitative Analysis of Cyclopropylfentanyl by LC-MS-MS
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DOI：
10.1093/jat/bky094
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发表时间：
2019-05-01
影响因子：
2.5
通讯作者：
Avella, Joseph
中科院分区：
文献类型：
--
作者：
Fagiola, Michael;Hahn, Timothy;Avella, Joseph
2018年1月，美国缉毒局暂时将环丙基芬太尼指定为附表I药物。在5个月的时间里（2017年12月至2018年5月），拿骚县法医毒理学实验室定性地鉴定并确认了从五个死后病例中获得的标本中的环丙基芬太尼。我们描述了这五个病例，包括相关的尸检结果和死者病史，以及死后心脏血液中环丙基芬太尼的检测结果。样品采用碱性液-液萃取法制备，样品pH调节为bbbb9，萃取溶剂为90:10己烷：乙酸乙酯。仪器分析通过液相色谱串联质谱法实现，双射流电喷雾源在正离子模式下工作。对每个感兴趣的分析物和内标进行两次离子跃迁监测。在报告的病例中，5例死后心脏血液标本中环丙基芬太尼的估计浓度范围为5.6至82 ng/mL。所有5例病例在确定的死因中都含有环丙基芬太尼。
In January 2018, the Drug Enforcement Agency temporarily designated cyclopropylfentanyl as a Schedule I drug. Over the course of 5 months (December 2017-May 2018), the Nassau County Medical Examiner Toxicology Laboratory qualitatively identified and confirmed cyclopropylfentanyl in specimens obtained from five postmortem cases. We describe the five cases and include pertinent autopsy findings and decedent histories, along with results for cyclopropylfentanyl determined in postmortem cardiac blood. Samples were prepared by an alkaline liquid-liquid extraction, with sample pH adjusted to >9 and utilizing an extraction solvent consisting of 90:10 hexane: ethyl acetate. Instrumental analysis was achieved via liquid chromatography tandem mass spectrometry with a dual jetstream electrospray source operating in positive ion mode. Two ion transitions were monitored for each analyte of interest and the internal standard. The estimated concentration range of cyclopropylfentanyl in the reported cases was 5.6 to 82 ng/mL for five postmortem cardiac blood specimens. All five cases included cyclopropylfentanyl in the established cause of death.