Changes in erythrocyte deformability in NaCl-induced right-sided cardiac failure in broiler chickens.
Changes in erythrocyte deformability in NaCl-induced right-sided cardiac failure in broiler chickens.
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DOI：
10.2460/ajvr.1992.53.12.2359
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发表时间：
1992-12
影响因子：
1
通讯作者：
Mirsalimi Sm;P. O'Brien;R. Julian
中科院分区：
文献类型：
--
作者：
Mirsalimi Sm;P. O'Brien;R. Julian
在这项研究中，我们验证了在氯化钠中毒所致心力衰竭的发展过程中红细胞变形性降低的假说。对饮用5g氯化钠/L的50只鸡中的6只在第7~42天测定了红细胞变形性和常规血液学、生化指标，并与自由饮用自来水的50只健康鸡中的6只进行了比较。通过将10%的红细胞悬浮液通过具有5微米孔的聚碳酸酯薄膜来评估变形性。对鸡实施安乐死，并测定心脏和体重。氯化钠治疗可导致右心衰竭，直至第28天。心脏重量与体重的比率更大，右室比高20%，左室比左室高15-27%，这可归因于氯化钠处理。经氯化钠处理的鸡红细胞变形性显著降低，与红细胞大小和血浆Na+浓度增加有关。然而，红细胞的肿胀只能解释变形性降低的一部分。变形能力的降低不能用细胞粘度的增加来解释，因为红细胞粘度的主要决定因素--平均红细胞血红蛋白浓度降低了。由于红细胞变形性降低与血管阻力增加有关，因此变形性降低可能是这些鸡发生右心衰的原因之一。
In this study, we tested the hypothesis that erythrocyte deformability is decreased in the development of cardiac failure induced by NaCl toxicosis. Deformability of erythrocytes and routine hematologic and biochemical variables were measured in 6 of 50 chickens that were given 5 g of NaCl/L in their drinking water from day 7 to day 42, and were compared with values in 6 of 50 healthy chickens given free access to tap water. Deformability was assessed by passing a 10% suspension of erythrocytes through a polycarbonate membrane with 5-microns pores. Chickens were euthanatized and heart and body weights were determined. Treatment with NaCl induced right-sided cardiac failure up to day 28. The ratios of heart weight to body weight were greater, for right ventricle by 20 to 64% and for left ventricle by 15 to 27%, attributable to NaCl treatment. Deformability of erythrocytes of NaCl-treated chickens was markedly decreased, in association with increased erythrocyte size and plasma Na+ concentration. However, only part of the decreased deformability could be explained by swelling of erythrocytes. Decreased deformability could not be explained by increased cell viscosity because mean corpuscular hemoglobin concentration, the primary determinant of erythrocyte viscosity, was decreased. Because decreased deformability of erythrocytes has been demonstrated previously to be associated with increased vascular resistance, decreased deformability may have contributed to the development of right-sided cardiac failure in these chickens.