A visual pigment from chicken that resembles rhodopsin: amino acid sequence, gene structure, and functional expression.
A visual pigment from chicken that resembles rhodopsin: amino acid sequence, gene structure, and functional expression.
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鸡的视觉色素，类似于视紫红质：氨基酸序列、基因结构和功能表达。
DOI：
10.1021/bi00128a002
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Nathans,J
中科院分区：
文献类型：
--
作者：
Wang,SZ;Adler,R;Nathans,J
威尔默眼科研究所，霍华德休斯医学研究所，分子生物学和遗传学系，神经科学系，约翰霍普金斯大学医学院，马里兰州巴尔的摩市21205年10月3日收到；摘要：通过对鸡视紫红质样视觉色素基因的分离和测序，确定了其氨基酸序列。预测的序列与牛、人类和鸡的视紫红质有70%到80%的相同，与人类的蓝色、绿色和红色视锥色素、鸡的红色视锥色素和洞穴鱼的长波视锥色素有40%到50%的相同。通过将cDNA转染到培养细胞中，编码色素在495nm处吸收最大，与50 mM羟胺在20℃下反应，半反应时间为16 min。这些特性，再加上氨基酸序列预测的高pI，表明该克隆基因编码之前通过生化和光谱研究确定的鸡绿色色素。这个序列定义了视觉色素基因家族的一个新分支。
The Wilmer Ophthalmological Institute, Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, and Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 Received October 3, 1991; Revised Manuscript Received December 30, 1991 abstract: The amino acid sequence of a rhodopsin-like visual pigment from chickens has been determined by isolating and sequencing its gene. The predicted sequence is between 70% and 80% identical tobovine, human, and chicken rhodopsins and between 40% and 50% identical to human blue, green, and red cone pigments, the chicken red cone pigment, and cavefish long-wave cone pigments. The encoded pigment, produced by transfection of cDNA into cultured cells, absorbs maximally at 495 nm as determined from photobleaching difference spectra and reacts at 20 C with 50 mM hydroxylamine with a half-time of 16 min. These properties, together with a high pI predicted from the amino acid sequence, suggest that this cloned gene encodes the chicken green pigment previously identified by biochemical and spectroscopic studies. This sequence defines a new branch of the visual pigment gene family.