Left-Right Symmetry or Asymmetry of Cells on Stripe-Like Micropatterned Material Surfaces
Left-Right Symmetry or Asymmetry of Cells on Stripe-Like Micropatterned Material Surfaces
复制标题
条纹状微图案材料表面上细胞的左右对称或不对称
DOI：
10.1002/cjoc.201800124
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发表时间：
2018-07-01
影响因子：
5.4
通讯作者：
Ding, Jiandong
中科院分区：
文献类型：
--
作者：
Hu, Yiwen;Yao, Xiang;Ding, Jiandong
材料表面可以诱导细胞反应，如接触引导，但对进一步的细胞定向关注较少。在这里，我们报告了一个有趣的现象，细胞取向超越了经典的接触指导，在一个条状微图案上，细胞粘附性精氨酸-甘氨酸-天冬氨酸(RGD)肽在聚乙二醇(PEG)装饰的无污染背景上。这种具有细胞粘附性对比的微图案在细胞种植后产生了显著的接触指导。此外，定位和拉长的细胞进一步定位于粘连条纹之外，甚至观察到大鼠间充质干细胞(RMSCs)呈逆时针方向旋转。RMSCs左右不对称仅有统计学意义，我们观察了所有病例，包括顺时针方向、逆时针方向或仅保持先前接触指导的方向。我们进一步发现，人包皮成纤维细胞(HFF)喜欢顺时针方向旋转，而人骨髓间充质干细胞(HMSCs)和人脐血管内皮细胞(HUVECs)对这两个方向都没有明显的偏好，这表明左右对称或不对称取决于细胞类型。本报告部分证实了细胞手性并揭示了其复杂性，呼吁进一步仔细和全面地研究材料表面的细胞手性这一具有挑战性的课题。
Material surfaces can induce cell responses such as contact guidance, yet little attention has been paid to further cell orientation. Herein, we report an interesting phenomenon of cell orientation beyond the classic contact guidance on a stripe-like micropattern with cell-adhesive arginine- glycine-aspartate (RGD) peptides on a nonfouling background decorated by poly(ethylene glycol) (PEG). Such a micropattern with cell adhesion contrast led to significant contact guidance after cell seeding. What is more, the localized and elongated cells were found to be further orientated out of the adhesive stripes, and even an anticlockwise rotation was observed for rat mesenchymal stem cells (rMSCs). The left-right asymmetry of rMSCs stood only in statistics, for we observed all cases including clockwise orientation, anticlockwise orientation or just keeping the orientation of previous contact guidance. We further found that human foreskin fibroblasts (HFFs) preferred a clockwise rotation, while human mesenchymal stem cells (hMSCs) and human umbilical vascular endothelial cells (HUVECs) exhibited no significant preference to either direction, which indicated that the left-right symmetry or asymmetry was cell-type dependent. The present report has partially confirmed the cell chirality and revealed its complexity, calling for further careful and comprehensive investigation of the challenging topic of cell chirality on material surfaces.