The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats
The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats
复制标题
脉冲电磁场对链脲佐菌素治疗大鼠糖尿病骨丢失的预防作用
DOI：
10.1007/s00198-010-1447-3
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发表时间：
2011-06-01
影响因子：
4
通讯作者：
Xu, Q.
中科院分区：
文献类型：
--
作者：
Jing, D.;Cai, J.;Xu, Q.
本研究首次证实脉冲电磁场(PEMF)能部分阻止链脲佐菌素诱导的糖尿病大鼠的骨强度和骨结构恶化，改善受损的骨形成。结果表明，PEMF可能成为一种潜在的抑制糖尿病骨量减少或骨质疏松的添加剂方法。糖尿病(DM)可引起各种肌肉骨骼的异常。糖尿病骨并发症的最佳治疗方法仍然缺乏。开发更有效、更安全的治疗糖尿病骨病的方法是非常必要的。脉冲电磁场(PEMF)作为一种替代的非侵入性方法，已被证明在非糖尿病条件下治疗骨折愈合和骨质疏松是有效的。然而，PEMF对糖尿病骨并发症的治疗作用尚未见报道，本文系统评价了PEMF对链脲佐菌素治疗的糖尿病大鼠骨丢失的预防作用。进行了两个类似的实验。在每个实验中，16只糖尿病大鼠和8只非糖尿病大鼠被等分成对照组、DM组和DM+PEMF组。在实验1中，三点弯曲试验表明，PEMF改善了糖尿病骨组织的生物力学质量，表现为最大载荷、刚度和能量吸收增加。微计算机断层扫描分析显示，PEMF可部分逆转DM所致的骨结构恶化，表现为Tb.N、Tb.Th、Bv/Tv和Cn.D增加，Tb.Sp和SMI降低。血清OC分析表明，PEMF部分阻止了DM诱导的骨形成减少。在实验2中，骨吸收标记物TRACP5b无显著差异。动态骨组织形态计量学参数BFR/BS和OC.N/BS进一步支持了上述生化结果。结果表明，PEMF可以部分防止DM所致的骨强度和骨结构恶化，并改善受损的骨形成。PEMF可能成为抑制糖尿病骨质疏松的一种潜在的添加剂方法。
The present study was the first report demonstrating that pulsed electromagnetic field (PEMF) could partially prevent bone strength and architecture deterioration and improve the impaired bone formation in streptozotocin-induced diabetic rats. The findings indicated that PEMF might become a potential additive method for inhibiting diabetic osteopenia or osteoporosis.Diabetes mellitus (DM) can cause various musculoskeletal abnormalities. Optimal therapeutic methods for diabetic bone complication are still lacking. It is essential to develop more effective and safe therapeutic methods for diabetic bone disorders. Pulsed electromagnetic field (PEMF) as an alternative noninvasive method has proven to be effective for treating fracture healing and osteoporosis in non-diabetic conditions. However, the issue about the therapeutic effects of PEMF on diabetic bone complication has not been previously investigated.We herein systematically evaluated the preventive effects of PEMF on diabetic bone loss in streptozotocin-treated rats. Two similar experiments were conducted. In each experiment, 16 diabetic and eight non-diabetic rats were equally assigned to the control, DM, and DM + PEMF group. DM + PEMF group was subjected to daily 8-h PEMF exposure for 8 weeks.In experiment 1, three-point bending test suggested that PEMF improved the biomechanical quality of diabetic bone tissues, evidenced by increased maximum load, stiffness, and energy absorption. Microcomputed tomography analysis demonstrated that DM-induced bone architecture deterioration was partially reversed by PEMF, evidenced by increased Tb.N, Tb.Th, BV/TV, and Conn.D and reduced Tb.Sp and SMI. Serum OC analysis indicated that PEMF partially prevented DM-induced decrease in bone formation. In experiment 2, no significant difference in the bone resorption marker TRACP5b was observed. These biochemical findings were further supported by the dynamic bone histomorphometric parameters BFR/BS and Oc.N/BS.The results demonstrated that PEMF could partially prevent DM-induced bone strength and architecture deterioration and improve the impaired bone formation. PEMF might become a potential additive method for inhibiting diabetic osteoporosis.