Circulating levels of Th1 and Th2 chemokines in patients with ankylosing spondylitis
Circulating levels of Th1 and Th2 chemokines in patients with ankylosing spondylitis
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强直性脊柱炎患者循环Th1和Th2趋化因子水平
DOI：
10.1016/j.cyto.2016.01.012
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发表时间：
2016-05-01
期刊：
影响因子：
3.8
通讯作者：
Yang, Pingting
中科院分区：
文献类型：
--
作者：
Wang, Jianing;Zhao, Qi;Yang, Pingting
目的：虽然趋化因子是炎症过程中选择性吸引和激活各种白细胞亚群的关键因素，但有关强直性脊柱炎（AS）中辅助性T细胞（Th）1或Th 2趋化因子的研究很少。本研究旨在确定血清趋化因子水平是否优先趋化Th 1（IFN-γ诱导蛋白-10，IP-10/CXCL 10）和Th 2（胸腺和活化调节趋化因子，TARC/CCL 17）和（巨噬细胞源性趋化因子，MDC/CCL 22）细胞的升高以及它们是否与AS患者的临床特征相关。42例轴型AS患者和25例健康对照者入组研究。使用ELISA检测趋化因子（IP-10、TARC和MDC）和细胞因子（IFN-γ、TNF-α和IL-4）的血清水平。采用强直性脊柱炎疾病活动度评分（ASDAS）评价疾病活动度。结果：AS患者血清趋化因子IP-10、TARC和MDC水平均显著高于健康对照组，且与正常对照组比较差异有统计学意义（P < 0. 05）。血清细胞因子IFN-γ、TNF-α水平也显著升高，但IL-4水平无明显变化。AS患者IP-10水平与ESP、CRP和ASDAS相关，而TARC和MDC水平与这些临床指标无关。趋化因子和细胞因子水平之间的相关性分析显示IP-10和TNF-α之间呈正相关。结论：Th 1趋化因子IP-10和Th 2趋化因子TARC和MDC在AS的发生发展中起协同作用。(C)2016爱思唯尔有限公司版权所有。
Objective: Although chemokines are critical elements for the selective attraction and activation of various leukocyte subsets in the inflammatory process, there are few findings concerning T helper (Th) 1 or Th2 chemokines in ankylosing spondylitis (AS). This study was designed to determine whether serum levels of chemokines that are preferentially chemotactic for Th1 (IFN-gamma-inducible protein-10, IP-10/ CXCL10) and Th2 (thymus and activation regulated chemokine, TARC/CCL17) and (macrophage derived chemokine, MDC/CCL22) cells were elevated and whether they correlated with the clinical features in patients with AS.Methods: Forty-two patients with axial AS and 25 healthy controls were enrolled into the study. Serum levels of chemokines (IP-10, TARC and MDC) and cytokines (IFN-gamma, TNF-alpha and IL-4) were examined using ELISA. The disease activity was evaluated by Ankylosing Spondylitis Disease Activity Score (ASDAS). Serum levels of IgG, IgA, IgM, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were measured.Results: Serum chemokine levels of IP-10, TARC and MDC were significantly higher in patients with AS than those in healthy controls. Serum cytokine levels of IFN-gamma, TNF-alpha were also significantly increased, but the levels of IL-4 were not. Furthermore, IP-10 levels in AS patients correlated with ESP, CRP and ASDAS, while the levels of TARC and MDC did not correlate with these clinic indexes. Correlation analysis between the levels of chemokines and cytokines revealed a positive correlation between IP-10 and TNF-alpha. The levels of both Th1 and Th2 chemokines decreased under blockade of TNF-alpha.Conclusion: Our results suggest that both a Th1 chemoattractant IP-10 and Th2 chemoattractants, TARC and MDC, cooperatively play a role in the development of AS. (C) 2016 Elsevier Ltd. All rights reserved.