Photoacoustic tomography imaging and estimation of oxygen saturation of hemoglobin in ocular tissue of rabbits
Photoacoustic tomography imaging and estimation of oxygen saturation of hemoglobin in ocular tissue of rabbits
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DOI：
10.1016/j.exer.2015.05.022
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发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Hennen, Stella N.;Xing, Wenxin;Wang, Lihong V.
本研究评价了声学分辨率（AR）光声断层扫描（PAT）定量监测兔睫状体组织血红蛋白氧饱和度（sO（2））的体内成像能力和安全性。AR PAT用于从七只新西兰白色兔的睫状体脉管系统收集经巩膜图像。PAT sO（2）测量值在以下条件下获得：当通过脉搏血氧仪测量的全身502在100%和99%之间（水平1）时;通过脉搏血氧仪测量的全身sO（2）在98%和90%之间（水平2）;通过脉搏血氧仪测量的全身sO（2）小于90%（水平3）。成像后，进行眼组织的组织学分析，以评价AR PAT成像引起的可能的结构损伤。AR PAT能够解析通过角膜或前巩膜观察的眼前段的解剖结构。组织学研究显示无眼部损伤。平均而言，AR PAT获得的sO（2）值（%）低于脉搏血氧仪获得的sO（2）值（所有p < 0.001）：水平1、2和3分别为86.28 +/- 4.16 vs 99.25 +/- 0.28、84.09 +/- 1.81 vs 95.3 +/- 2.6和64.49 +/- 7.27 vs 71.15 +/- 10.21。AR PAT成像模式能够对家兔深部组织sO（2）进行定性监测。需要进一步的研究来验证和修改AR PAT模态，特别是用于人眼。安全、无创的眼前节sO（2）活体成像方法对于评价氧化损伤、缺氧和缺血在眼部疾病发病机制中的作用的研究具有重要意义。爱思唯尔有限公司出版
This study evaluated in vivo imaging capabilities and safety of qualitative monitoring of oxygen saturation of hemoglobin (sO(2)) of rabbit ciliary body tissues obtained with acoustic resolution (AR) photoacoustic tomography (PAT). AR PAT was used to collect trans-scleral images from ciliary body vasculature of seven New Zealand White rabbits. The PAT sO(2) measurements were obtained under the following conditions: when systemic 502 as measured by pulse oximetry was between 100% and 99% (level 1); systemic sO(2) as measured by pulse oximetry was between 98% and 90% (level 2); and systemic sO(2) as measured by pulse oximetry was less than 90% (level 3). Following imaging, histological analysis of ocular tissue was conducted to evaluate for possible structural damage caused by the AR PAT imaging. AR PAT was able to resolve anatomical structures of the anterior segment of the eye, viewed through the cornea or anterior sclera. Histological studies revealed no ocular damage. On average, sO(2) values (%) obtained with AR PAT were lower than sO(2) values obtained with pulse oximetry (all p < 0.001): 86.28 +/- 4.16 versus 99.25 +/- 0.28, 84.09 +/- 1.81 vs. 95.3 +/- 2.6, and 64.49 +/- 7.27 vs. 71.15 +/- 10.21 for levels 1, 2 and 3 respectively. AR PAT imaging modality is capable of qualitative monitoring for deep tissue sO(2) in rabbits. Further studies are needed to validate and modify the AR PAT modality specifically for use in human eyes. Having a safe, non-invasive method of in vivo imaging of sO(2) in the anterior segment is important to studies evaluating the role of oxidative damage, hypoxia and ischemia in pathogenesis of ocular diseases. Published by Elsevier Ltd.