The rise of neutron cryo-crystallography.
The rise of neutron cryo-crystallography.
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DOI：
10.1107/s205979831800640x
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发表时间：
2018-08-01
期刊：
影响因子：
--
通讯作者：
Moody PCE
中科院分区：
文献类型：
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作者：
Kwon H;Langan PS;Coates L;Raven EL;Moody PCE
讨论了蛋白质X射线晶体学中低温数据采集环境在中子蛋白质晶体学中的应用。在X射线数据收集过程中，使用汽化液氮将蛋白质晶体保持在100 K几乎已变得普遍。将此应用于中子蛋白质晶体学提供了机会，以显着扩大范围的生化问题，可以解决，虽然必须小心，在假设直接外推到室温总是有效的。在这里，中子蛋白质冷冻结晶学的历史，并与中子晶体学所需的较大的晶体的安装和低温冷却的特定问题和解决方案进行了审查。最后，进一步的低温中子研究，利用现有的和未来的中子仪器的前景进行了讨论。
The application of the cryogenic data-collection environments used in protein X-ray crystallography to neutron protein crystallography is discussed. The use of boiled-off liquid nitrogen to maintain protein crystals at 100 K during X-ray data collection has become almost universal. Applying this to neutron protein crystallography offers the opportunity to significantly broaden the scope of biochemical problems that can be addressed, although care must be taken in assuming that direct extrapolation to room temperature is always valid. Here, the history to date of neutron protein cryo-crystallography and the particular problems and solutions associated with the mounting and cryocooling of the larger crystals needed for neutron crystallography are reviewed. Finally, the outlook for further cryogenic neutron studies using existing and future neutron instrumentation is discussed.