Solubilization of membrane bound opiate receptor from rat brain.
Solubilization of membrane bound opiate receptor from rat brain.
复制标题
大鼠脑膜结合阿片受体的溶解。
DOI:
10.1016/0024-3205(81)90723-2
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发表时间:
1981
期刊:
影响因子:
6.1
通讯作者:
Loh,HH
中科院分区:
文献类型:
--
作者:
Cho,TM;Yamato,C;Cho,JS;Loh,HH
Sonication of rat brain membranes for 9 minutes solubilized 35% of their stereospecific opiate binding activity; a second 9 minute sonication of the insoluble residue released an additional 21% of the original binding. The opiate binding properties of the solubilized material were highly similar to those of membrane bound receptor by a number of criteria, including affinity, effect of sodium, and the IC50of unlabeled opiates in displacing3H-etorphine binding. Moreover, storage of the solubilized receptor fraction for two weeks at −20°C did not significantly change the receptor binding. Sonication thus appears to be a useful first step in purifying the opiate receptor.
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DOI:
10.1111/j.1600-0773.1973.tb01477.x
发表时间:
2009
期刊:
Acta pharmacologica et toxicologica
影响因子:
--
作者:
L. Terenius
通讯作者:
L. Terenius
影响因子:
5
作者:
U. Ruegg;J. Hiller;E. J. Simon
通讯作者:
E. J. Simon
影响因子:
56.9
作者:
E. J. Simon;J. Hiller;I. Edelman
通讯作者:
I. Edelman
影响因子:
56.9
作者:
R. Guillery;G. Scott;B. Cattanach;M. Deol
通讯作者:
M. Deol
DOI:
--
发表时间:
1980
期刊:
Life Science
影响因子:
--
作者:
Jean M. Bidlack;L. Abood
通讯作者:
L. Abood