Solubilization of membrane bound opiate receptor from rat brain.

Solubilization of membrane bound opiate receptor from rat brain.
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大鼠脑膜结合阿片受体的溶解。

DOI:
10.1016/0024-3205(81)90723-2
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发表时间:
1981
期刊:
影响因子:
6.1
通讯作者:
Loh,HH
Loh,HH
中科院分区:
医学2区
文献类型:
--
作者:
Cho,TM;Yamato,C;Cho,JS;Loh,HH

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Sonication of rat brain membranes for 9 minutes solubilized 35% of their stereospecific opiate binding activity; a second 9 minute sonication of the insoluble residue released an additional 21% of the original binding. The opiate binding properties of the solubilized material were highly similar to those of membrane bound receptor by a number of criteria, including affinity, effect of sodium, and the IC50of unlabeled opiates in displacing3H-etorphine binding. Moreover, storage of the solubilized receptor fraction for two weeks at −20°C did not significantly change the receptor binding. Sonication thus appears to be a useful first step in purifying the opiate receptor.
麻醉镇痛药与大鼠大脑皮层膜部分突触质之间的立体特异性相互作用。
DOI: 10.1111/j.1600-0773.1973.tb01477.x
发表时间: 2009
期刊: Acta pharmacologica et toxicologica
影响因子: --
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DOI: --
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大鼠脑中立体特异性阿片大分子复合物的溶解
DOI: --
发表时间: 1975
期刊: Science
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DOI: --
发表时间: 1973
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