The mouse Clock mutation behaves as an antimorph and maps within the W19H deletion, distal of Kit.
The mouse Clock mutation behaves as an antimorph and maps within the W19H deletion, distal of Kit.
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发表时间：
1997-07
期刊：
影响因子：
3.3
通讯作者：
D. P. King;M. Vitaterna;Anne-Marie Chang;W. Dove;Lawrence H. Pinto;F. Turek;J. Takahashi
中科院分区：
文献类型：
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作者：
D. P. King;M. Vitaterna;Anne-Marie Chang;W. Dove;Lawrence H. Pinto;F. Turek;J. Takahashi
Clock是一种半显性突变，从小鼠的N-乙基-N-亚硝脲诱变筛选中鉴定出来。携带时钟突变的小鼠表现出昼夜行为的异常，包括内源性周期的延长和节律性的丧失。为了确定受这种突变影响的基因，我们已经生成了时钟基因座的高分辨率遗传图谱(>；1800个减数分裂)。我们报道了Clock位于小鼠5号染色体Kit的0.7 cM远端。定位结果表明，Clock位于W19H缺失区域内。不同CLOCK和W19H复合基因的互补分析表明，CLOCK突变表现为一种反晶型。CLOCK的这种反变形行为有力地证明了CLOCK定义了一个与哺乳动物昼夜节律系统密切相关的基因。
Clock is a semidominant mutation identified from an N-ethyl-N-nitrosourea mutagenesis screen in mice. Mice carrying the Clock mutation exhibit abnormalities of circadian behavior, including lengthening of endogenous period and loss of rhythmicity. To identify the gene affected by this mutation, we have generated a high-resolution genetic map (> 1800 meioses) of the Clock locus. We report that Clock is 0.7 cM distal of Kit on mouse chromosome 5. Mapping shows that Clock lies within the W19H deletion. Complementation analysis of different Clock and W19H compound genotypes indicates that the Clock mutation behaves as an antimorph. This antimorphic behavior of Clock strongly argues that Clock defines a gene centrally involved in the mammalian circadian system.