Isolated hepatic granulomas from mice infected with Schistosoma mansoni contain nerve growth factor.
Isolated hepatic granulomas from mice infected with Schistosoma mansoni contain nerve growth factor.
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曼氏血吸虫感染小鼠的分离肝肉芽肿含有神经生长因子。
DOI：
10.1128/iai.59.12.4443-4449.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Pantazis,NJ
中科院分区：
文献类型：
--
作者：
Varilek,GW;Weinstock,JV;Pantazis,NJ
神经生长因子（NGF）是一种神经营养因子，对特定神经元细胞群的发育和维持至关重要。此外，NGF对炎症细胞具有生物学作用。本研究的目的是确定是否存在神经生长因子在慢性炎症，使用分离的肝肉芽肿感染的小鼠曼氏血吸虫作为模型。肉芽肿是一种复杂的T细胞介导的对虫卵的免疫反应。从感染小鼠的肝脏中分离出完整的肉芽肿，并检查是否存在NGF。在均质化的肉芽肿样品中，放射免疫分析和免疫印迹分析检测到具有与纯化的鼠神经生长因子（13 kDa）相同分子量的免疫反应性神经生长因子。在大鼠嗜铬细胞瘤（PC12）生物测定中，体外培养的分离肉芽肿释放具有NGF样神经突促进活性的可溶性因子。该活性被阻断性抗NGF抗体部分抑制。有两个潜在的来源，这种神经生长因子样神经突促进活动，无论是卵细胞或宿主的炎症反应。由于分离的卵和可溶性卵抗原都没有促进神经突的活性，因此炎症是这种活性的来源。在生物测定中，抗NGF抗体不能完全抑制肉芽肿诱导的神经突生长，这表明NGF不是这些肉芽肿中存在的唯一神经营养因子。肉芽肿内存在NGF可能表明NGF在肉芽肿反应中起作用。
Nerve growth factor (NGF) is a neurotrophic factor essential for the development and maintenance of specific neuronal cell populations. In addition, NGF has biological effects on inflammatory cells. The aim of this study was to determine whether NGF is present in chronic inflammation, using isolated hepatic granulomas from mice infected with Schistosoma mansoni as the model. The schistosome granuloma is a complex T-cell-mediated immune response to the egg. Intact granulomas were isolated from the livers of infected mice and examined for the presence of NGF. In homogenized granuloma samples, radioimmunoassay and immunoblotting analyses detected an immunoreactive NGF that had the same molecular mass as that of purified murine NGF (13 kDa). Isolated granulomas cultured in vitro released soluble factor(s) with NGF-like neurite-promoting activity in a rat pheochromocytoma (PC12) bioassay. This activity was partially inhibited by a blocking anti-NGF antibody. There were two potential sources of this NGF-like neurite-promoting activity, either the schistosome egg or the host inflammatory response. Since neither isolated eggs nor soluble egg antigen had neurite-promoting activity, the inflammation was the source of this activity. The inability of the anti-NGF antibody to inhibit completely the granuloma-induced neurite outgrowth in the bioassay signifies that NGF is not the only neurotrophic factor present in these granulomas. The presence of NGF within the granulomas may indicate that NGF has a role in the granulomatous response.