Lineage segregation, pluripotency and X-chromosome inactivation in the pig pre-gastrulation embryo
Lineage segregation, pluripotency and X-chromosome inactivation in the pig pre-gastrulation embryo
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DOI：
10.1101/347823
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
P. Ramos-Ibeas;Fei Sang;Qifan Zhu;Walfred W. C. Tang;Sarah Withey;Doris Klisch;M. Loose;M. Surani;R. Alberio
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文献类型：
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作者：
P. Ramos-Ibeas;Fei Sang;Qifan Zhu;Walfred W. C. Tang;Sarah Withey;Doris Klisch;M. Loose;M. Surani;R. Alberio
最近出现了描绘哺乳动物胚胎发生的细胞基础的高分辨率分子程序。对人类胚胎的类似分析仅限于植入前阶段，因为植入后早期胚胎无法获得。尽管如此，我们以前提出了猪和人类早期发育的保守原则。为了进一步了解多能状态和谱系划分，我们分析了单细胞分辨率的猪胚胎。在这里，我们显示了内细胞团和滋养外胚层在早期囊胚，然后在晚期囊胚的上胚层和下胚层进行性分离。我们检测到不同的多能状态，首先是短暂的“幼稚”状态，然后是长期的启动状态。通过晚期外胚层中的X-失活，可获得雌性X染色体的剂量补偿。详细的人-猪比较是理解人类早期发育的基础，也是进一步研究猪种间嵌合体中人多能干细胞分化的基础。
High-resolution molecular programs delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and then of epiblast and hypoblast in late blastocysts. We detected distinct pluripotent states, first as a short ‘naïve’ state followed by a protracted primed state. Dosage compensation with respect to the X-chromosome in females is attained via X-inactivation in late epiblasts. Detailed human-pig comparison is a basis towards comprehending early human development and a foundation for further studies of human pluripotent stem cell differentiation in pig interspecies chimeras.