ACCUMULATION OF ORGANIC ACIDS BY HELA CELLS INFECTED WITH TYPE-4 ADENOVIRUS
ACCUMULATION OF ORGANIC ACIDS BY HELA CELLS INFECTED WITH TYPE-4 ADENOVIRUS
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DOI：
10.3181/00379727-95-23113
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发表时间：
1957-01-01
影响因子：
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通讯作者：
GINSBERG, HS
中科院分区：
文献类型：
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作者：
FISHER, TN;GINSBERG, HS
感染4型腺病毒的培养液比未感染的对照培养液明显更酸。受感染的培养物的pH值降低是由于乳酸、乳酸、乙酸和α-酮基谷氨酸的积累。采用硅藻土柱层析分离，比色法和酸碱滴定法测定。通过纸色谱法确认有机酸的身份。伴随着感染的培养物的液体中的酸的积累增加，病毒感染的培养物的细胞对葡萄糖的利用增加。
Fluids from cultures infected with type 4 adeno-virus were markedly more acid than fluids from non-infected control cultures. The lowered pH of infected cultures resulted from an accumulation of lactic, pyruvic, acetic and alpha ketoglutaric acids. The acids were separated by chromatography on a celite column, and measured by colorimetric and acid-base titration technics. The identity of the organic acids was confirmed by paper chromatography. Concomitant with the increased accumulation of acid in fluids of infected cultures, an increased utilization of glucose by cells of the virus-infected cultures occurred.