Unreduced diploid sperm from diploid hybrids and formation of a new type of tetraploid hybrid
Unreduced diploid sperm from diploid hybrids and formation of a new type of tetraploid hybrid
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来自二倍体杂种的未还原二倍体精子和新型四倍体杂种的形成
DOI：
10.1016/j.aquaculture.2019.734584
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发表时间：
2020-01
期刊：
影响因子：
4.5
通讯作者：
Shaojun Liu
中科院分区：
文献类型：
--
作者：
Yude Wang;Minghe Zhang;Suifei Tao;Xiang Xie;Huifang Tan;Liu Cao;Jing Wang;Qinbo Qin;Chun Zhang;Min Tao;Ming Ma;Bo Chen;Shaojun Liu
未减数配子的产生是多倍体形成的重要来源。本研究以锦鲤（KOC; 2n = 100; KOC）×彩鲫（CCC; 2n = 100; KCC）为亲本，获得了具有不减数二倍体精子的第二代二倍体杂种（2nKCF 2）。与KOC和CCC相比，2nKCF 2具有独特的形态特征，包括灰色和金色的头部，金棕色鳞片状外缘，光滑的金黄色背部。FISH和5S rDNA分析表明，2nKCF 2不仅遗传了双亲的基因组DNA，而且丢失了部分双亲DNA片段。生殖细胞的染色体扩散提供了直接的细胞学证据的生产未减少的二倍体精子的男性2nKCF 2。电镜结果显示，2nKCF 2产生的二倍体精子头部比CCC产生的单倍体精子头部大一倍（P < 0.05），证实2nKCF 2产生的精子为二倍体。以2nKCF 2为父本，与改良的红鲫×鲤异源四倍体母本杂交，获得了一种新型的四倍体杂种（4 n = 200）。这项研究为生殖生物学和水产养殖提供了见解。
The production of unreduced gametes is an important resource for the formation of polyploids. Here, we obtained a second generation of diploid hybrids (2nKCF2) derived from koi carp (KOC; 2n = 100; ♀) × colored crucian carp (CCC; 2n = 100; ♂) with the unusual reproductive trait of producing unreduced diploid sperm. Compared with KOC and CCC, 2nKCF2had unique morphological characteristics, including a gray and golden head, golden-brown scaly outer edge, and smooth golden-yellow back. FISH and 5S rDNA analysis revealed that 2nKCF2not only inherited genomic DNA from their parents but also lost some parental DNA fragments. Chromosomal spreads of germ cells provided direct cytological evidence for the production of unreduced diploid sperm by male 2nKCF2. Electron microscopy results showed that the heads of the diploid sperm generated by 2nKCF2were twice as large as those of the haploid sperm generated by CCC (P < 0.05), confirming that the sperm of 2nKCF2were diploid. A new type of tetraploid hybrid (4n = 200) was produced by crossing 2nKCF2males with improved female allotetraploid hybrids of red crucian carp (♀) × common carp (♂). This study provides insights into reproductive biology and aquaculture.
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影响因子：
5.3
作者：
Mason AS;Nelson MN;Yan G;Cowling WA
通讯作者：
Cowling WA
影响因子：
3.1
作者：
PERRY, MC;MCINTOSH, MS;WELTERLEN, M
通讯作者：
WELTERLEN, M
DOI：
--
发表时间：
1981
期刊：
--
影响因子：
--
作者：
F. Schwartz
通讯作者：
F. Schwartz
影响因子：
3.8
作者：
D. P. Biradar;A. Rayburn
通讯作者：
D. P. Biradar;A. Rayburn
影响因子：
3.8
作者：
K. Morishima;K. Morishima;Hiroyuki Yoshikawa;K. Arai
通讯作者：
K. Morishima;K. Morishima;Hiroyuki Yoshikawa;K. Arai