The reliability and validity of hand-held refractometry water content measures of hydrogel lenses.
The reliability and validity of hand-held refractometry water content measures of hydrogel lenses.
复制标题
手持式折射仪水凝胶镜片含水量测量的可靠性和有效性。
DOI：
10.1097/00006324-200306000-00011
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Good,GregoryW
中科院分区：
文献类型：
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作者：
Nichols,JasonJ;Mitchell,GLynn;Good,GregoryW
目的。方法：19个不同标称含水量的晶状体，由两名验光员分两次检测，时间间隔为1小时。所有水含量测量均使用Atago N2手持式折光计。镜片由第三方以随机顺序呈现给每个审查员，审查员对任何潜在的镜片标识都是掩饰的。使用类内相关系数（ICC）、95%一致限和Wilcoxon符号秩检验来表征水晶体含水量测量的检验人内部和检验人之间的信度和效度。检查者内部信度非常好（ICC, 0.97； 95%一致限，−3.6%至+ 5.7%），访间平均差异为1.1±2.4%，无偏倚（p= 0.08）。审查员之间的信度也很好（ICC, 0.98； 95%的一致性限，−4.1%至+ 3.9%）。检查员之间的平均差异为- 0.1±2.1%（p= 0.83）。名义上报告的含水量与我们测量的含水量之间的平均差异为- 2.1±1.7%(p< 0.001)；该差异的95%一致性限为- 5.4% ~ + 1.1%。在测定水凝胶镜片的含水量时，检测人员内部和检测人员之间具有良好的可靠性。然而，在我们的镜片样品中，审查员往往高估了水凝胶镜片的标称含水量。如前所述，这种偏差可能与折射法中使用的白利度有关，并且与材料有关。因此，研究人员可能需要在通过手持式折射仪测量水凝胶透镜含水量时考虑偏差。
Purpose.To investigate within-and between-examiner reliability and validity of hand-held refractometry water content measures of hydrogel lenses.Methods.Nineteen lenses of various nominal water contents were examined by two examiners on two occasions separated by 1 hour. An Atago N2 hand-held refractometer was used for all water content measures. Lenses were presented in a random order to each examiner by a third party, and examiners were masked to any potential lens identifiers. Intraclass correlation coefficients (ICC), 95% limits of agreement, and Wilcoxon signed rank test were used to characterize the within-and between-examiner reliability and validity of lens water content measures.Results.Within-examiner reliability was excellent (ICC, 0.97; 95% limits of agreement,− 3.6% to+ 5.7%), and the inter-visit mean difference of 1.1±2.4% was not biased (p= 0.08). Between-examiner reliability was also excellent (ICC, 0.98; 95% limits of agreement,− 4.1% to+ 3.9%). The mean difference between examiners was− 0.1±2.1%(p= 0.83). The mean difference between the nominally reported water content and our water content measures was− 2.1±1.7%(p< 0.001); the 95% limits of agreement for this difference were− 5.4% to+ 1.1%.Conclusion.There is good reliability within and between examiners in measuring water content of hydrogel lenses. However, with our sample of lenses, examiners tended to overestimate the nominal water content of hydrogel lenses. As discussed, this bias may be associated with the Brix scale used in refractometry and is material dependent. Therefore, investigators may need to account for bias when measuring hydrogel lens water content via hand-held refractometry.