Fluorescence endomicroscopy with structured illumination
Fluorescence endomicroscopy with structured illumination
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DOI：
10.1364/oe.16.008016
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发表时间：
2008-05-26
期刊：
影响因子：
3.8
通讯作者：
Mertz, Jerome
中科院分区：
文献类型：
--
作者：
Bozinovic, Nenad;Ventalon, Cathie;Mertz, Jerome
我们提出了一种内窥镜装置，该装置利用结构化照明在约240 μ m的视野范围内产生高分辨率（类似于2.6 μ m）的光学切片荧光图像。内窥镜是基于使用柔性成像纤维束和小型化物镜。我们还提出了一种策略，可以在很大程度上抑制在厚组织中成像时出现的结构化照明伪影，这些组织显示出明显的失焦背景。为了确定我们的内窥镜在临床前或临床应用的潜力，我们提供了BCECF-AM标记的大鼠结肠粘膜图像。
We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (similar to 2.6 mu m) optically sectioned fluorescence images over a field of view of about 240 mu m. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifacts that arise when imaging in thick tissue that exhibits significant out-of-focus background. To establish the potential of our endomicroscope for preclinical or clinical applications, we provide images of BCECF-AM labeled rat colonic mucosa.