Inhibition of N-linked complex oligosaccharide formation by 1-deoxynojirimycin, an inhibitor of processing glucosidases.
Inhibition of N-linked complex oligosaccharide formation by 1-deoxynojirimycin, an inhibitor of processing glucosidases.
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DOI：
10.1016/s0021-9258(19)45358-1
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发表时间：
1982-12
期刊：
影响因子：
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通讯作者：
Brigitte Saunier;R. Kilker;Jan S. Tkaczq;Andrea Quaronill;A. Herscovics
中科院分区：
文献类型：
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作者：
Brigitte Saunier;R. Kilker;Jan S. Tkaczq;Andrea Quaronill;A. Herscovics
从酿酒酵母X-2180出发，在不加洗涤剂的情况下，以可溶性形式获得了从GlcZMangGlcNAcz和GlclManyGlcNAcz寡糖中去除葡萄糖残基的葡萄糖苷酶活性。这两种酶的活性显然是从GlcsMangGlcNAcz寡糖葡萄糖苷酶中分离出来的，该酶能从GlcsMan9GlcNAcz寡糖(Kilker，RD，Jr.，Saunier，B.，Tkacz，JS和Herskvics，A.(1981)J.Biol)中去除末端葡萄糖残基。化学。256,5299-5303)和作用于对-硝基苯基吡喃葡萄糖苷的a-和b-葡萄糖苷酶。GlcZMan9GlcNAcz和GlclMan9GlcNAcz的活性具有相同的性质，并且被葡萄糖以及所测试的各种a-和/3连接的葡萄糖二糖都被黑糖和麦芽糖所抑制。而GlcsMan9GlcNAcz寡糖葡萄糖苷酶不受葡萄糖的影响，但被曲二糖抑制。这些结果表明，酿酒酵母中存在两种特定的α-葡萄糖苷酶，负责低聚糖的加工。
Glucosidase activities which remove glucose residues from GlcZMangGlcNAcz and GlclManyGlcNAcz oligosaccharides were obtained in soluble form from Saccharomyces cerevisiae X-2180 without detergent. These two enzyme activities were clearly separated from the GlcsMangGlcNAcz oligosaccharide glucosidase which was shown previously to remove the terminal glucose residue from GlcsMan9GlcNAcz oligosaccharide (Kilker, RD, Jr., Saunier, B., Tkacz, JS, and Herscovics, A.(1981) J. Biol. Chem. 256, 5299-5303) and from the a-and b-glucosidases which act on p-nitrophenylglucopyranosides. The activities with both GlcZMan9GlcNAcz and GlclMan9GlcNAcz had the same properties and were inhibited to the same extent by glucose and, of various a-and/3-linked glucose disaccharides tested, by both nigerose and maltose. In contrast, the GlcsMan9GlcNAcz oligosaccharide glucosidase was not affected by glucose, but it was inhibited by kojibiose. These results suggest that there are two specific a-glucosidases responsible for oligosaccharide processing in S. cerevisiae.